Dengue viruses and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection
Open Access
- 1 July 1977
- journal article
- review article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 146 (1), 218-229
- https://doi.org/10.1084/jem.146.1.218
Abstract
Studies were made on the identity of human and monkey mononuclear leukocytes permissive to antibody-enhanced dengue 2 virus (D2V) infection. In cultures of peripheral blood leukocytes (PBL) inoculated immediately after separation, it was concluded that only mononuclear phagocytes support dengue infection. This is based upon observations that D2V-permissive cells were resistant to 1,200 rads, were both plastic adherent and nonadherent, were removed when passed through nylon wool columns in 10 percent fetal bovine serum or 100 percent autologous serum, and were destroyed by incubation with 100 mug/ml particulate silica. On direct immunofluorescence staining, perinuclear dengue antigen was visualized at 24 h, becoming maximal at 60 h. Antigen-containing cells had ample cytoplasm, ruffled cytoplasmic membrane, and 73 percent were actively phagocytic. As further evidence of the infection of mononuclear phagocytes, antibody-enhanced D2V replication was observed in bone marrow cultures from five of five rhesus monkeys, but not in cell cultures of spleen, thymus, or lymph nodes prepared from the same animals. It is hypothesized that dengue virus complexed with non-neutralizing antibody is internalized by immune phagocytosis in a mononuclear phagocyte with a defective virus-destroying mechanism. Dengue permissiveness may depend upon cellular immaturity since bone marrow leukocytes could be infected even when held for 4 days before infection while PBL held for this time decreased in permissiveness. In vitro antibody-dependent infection of mononuclear phagocytes should prove useful as a model for study of immunopathologic mechanisms in human dengue.Keywords
This publication has 12 references indexed in Scilit:
- Dengue viruses and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibodyThe Journal of Experimental Medicine, 1977
- Replication of Dengue-2 Virus in Cultured Human Lymphoblastoid Cells and Subpopulations of Human Peripheral LeukocytesThe Journal of Immunology, 1976
- Identification and characterization of the monoblast in mononuclear phagocyte colonies grown in vitro.The Journal of Experimental Medicine, 1975
- Dengue Carrier Culture and Antigen Production in Human Lymphoblastoid LinesIntervirology, 1975
- STUDIES OF THE HUMAN LYMPHOCYTE RECEPTOR FOR HEAT-AGGREGATED OR ANTIGEN-COMPLEXED IMMUNOGLOBULINThe Journal of Experimental Medicine, 1974
- Characterization of Glass Adherent Human Mononuclear CellsThe Journal of Immunology, 1973
- Immunological enhancement of dengue virus replication.1973
- The Development of Macrophages from Large Mononuclear Cells in the Blood of Patients with Inflammatory DiseaseJCI Insight, 1972
- The role of immunoglobulins in lymphocyte-mediated cell damage, in vitro. I. Comparison of the effects of target cell specific antibody and normal serum factors on cellular damage by immune and non-immune lymphocytes.1970
- THE ORIGIN OF MACROPHAGES FROM BONE MARROW IN THE RAT.1965