Adenosine Receptor–Mediated Adhesion of Endothelial Progenitors to Cardiac Microvascular Endothelial Cells
- 15 February 2008
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Circulation Research
- Vol. 102 (3), 356-363
- https://doi.org/10.1161/circresaha.107.158147
Abstract
Intracoronary delivery of endothelial progenitor cells (EPCs) is an emerging concept for the treatment of cardiovascular disease. Enhancement of EPC adhesion to vascular endothelium could improve cell retention within targeted organs. Because extracellular adenosine is elevated at sites of ischemia and stimulates neovascularization, we examined the potential role of adenosine in augmenting EPC retention to cardiac microvascular endothelium. Stimulation of adenosine receptors in murine embryonic EPCs (eEPCs) and cardiac endothelial cells (cECs) rapidly, within minutes, increased eEPC adhesion to cECs under static and flow conditions. Similarly, adhesion of human adult culture-expanded EPCs to human cECs was increased by stimulation of adenosine receptors. Furthermore, adenosine increased eEPC retention in isolated mouse hearts perfused with eEPCs. We determined that eEPCs and cECs preferentially express functional A1 and A2B adenosine receptor subtypes, respectively, and that both subtypes are involved in the regulation of eEPC adhesion to cECs. We documented that the interaction between P-selectin and its ligand (P-selectin glycoprotein ligand-1) plays a role in adenosine-dependent eEPC adhesion to cECs and that stimulation of adenosine receptors in cECs induces rapid cell surface expression of P-selectin. Our results suggest a role for adenosine in vasculogenesis and its potential use to stimulate engraftment in cell-based therapies.Keywords
This publication has 29 references indexed in Scilit:
- Bone-marrow-derived cells for cardiac stem cell therapy: safe or still under scrutiny?Nature Clinical Practice Cardiovascular Medicine, 2007
- Role of Adenosine Receptors in the Regulation of Angiogenic Factors and Neovascularization in HypoxiaThe Journal of pharmacology and experimental therapeutics, 2006
- Differential regulation of endothelial exocytosis of P-selectin and von Willebrand factor by protease-activated receptors and cAMPBlood, 2006
- Embryonic endothelial progenitor cells expressing a broad range of proangiogenic and remodeling factors enhance vascularization and tissue recovery in acute and chronic ischemiaThe FASEB Journal, 2005
- Endothelial Progenitor Cells Are Rapidly Recruited to Myocardium and Mediate Protective Effect of Ischemic Preconditioning via “Imported” Nitric Oxide Synthase ActivityCirculation, 2005
- Blood donor white blood cell reduction filters as a source of human peripheral blood-derived endothelial progenitor cellsTransfusion, 2005
- Assessment of the Tissue Distribution of Transplanted Human Endothelial Progenitor Cells by Radioactive LabelingCirculation, 2003
- Mast Cell–Mediated Stimulation of AngiogenesisCirculation Research, 2003
- Selectin GMP-140 (CD62; PADGEM) binds to sialosyl-Lea and sialosyl-Lex, and sulfated glycans modulate this bindingBiochemical and Biophysical Research Communications, 1991
- The effect of long-term vasodilatation on capillary growth and performance in rabbit heart and skeletal muscleCardiovascular Research, 1984