Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
Open Access
- 8 November 2010
- journal article
- Published by Springer Science and Business Media LLC in Journal of Experimental & Clinical Cancer Research
- Vol. 29 (1), 144-9
- https://doi.org/10.1186/1756-9966-29-144
Abstract
Real-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity. The gene expression is quantified by normalization to one or more reference genes which are presumed stably expressed throughout a given experiment. The aim of this study was to validate a standardized experimental setup to identifying reference genes for normalization of qRT-PCR in the metastatic and non-metastatic colon cancer.Keywords
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