Subinhibitory Arsenite Concentrations Lead to Population Dispersal in Thiomonas sp.
Open Access
- 18 August 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (8), e23181
- https://doi.org/10.1371/journal.pone.0023181
Abstract
Biofilms represent the most common microbial lifestyle, allowing the survival of microbial populations exposed to harsh environmental conditions. Here, we show that the biofilm development of a bacterial species belonging to the Thiomonas genus, frequently found in arsenic polluted sites and playing a key role in arsenic natural remediation, is markedly modified when exposed to subinhibitory doses of this toxic element. Indeed, arsenite [As(III)] exposure led to a considerable impact on biofilm maturation by strongly increasing the extracellular matrix synthesis and by promoting significant cell death and lysis within microcolonies. These events were followed by the development of complex 3D-biofilm structures and subsequently by the dispersal of remobilized cells observed inside the previously formed hollow voids. Our results demonstrate that this biofilm community responds to arsenite stress in a multimodal way, enhancing both survival and dispersal. Addressing this complex bacterial response to As(III) stress, which might be used by other microorganisms under various adverse conditions, may be essential to understand how Thiomonas strains persist in extreme environments.This publication has 28 references indexed in Scilit:
- Metabolic diversity among main microorganisms inside an arsenic-rich ecosystem revealed by meta- and proteo-genomicsThe ISME Journal, 2011
- Temporal transcriptomic response during arsenic stress in Herminiimonas arsenicoxydansBMC Genomics, 2010
- Structure, Function, and Evolution of the Thiomonas spp. GenomePLoS Genetics, 2010
- Cannibalism enhances biofilm development in Bacillus subtilisMolecular Microbiology, 2009
- Carbon and arsenic metabolism in Thiomonas strains: differences revealed diverse adaptation processesBMC Microbiology, 2009
- Formation ofStreptococcus pneumoniaeNon-Phase-Variable Colony Variants Is Due to Increased Mutation Frequency Present under Biofilm Growth ConditionsJournal of Bacteriology, 2008
- Endogenous oxidative stress produces diversity and adaptability in biofilm communitiesProceedings of the National Academy of Sciences of the United States of America, 2008
- Ecological Advantages of Autolysis during the Development and Dispersal of Pseudoalteromonas tunicata BiofilmsApplied and Environmental Microbiology, 2006
- New methodology for viability testing in environmental samplesMolecular and Cellular Probes, 2006
- Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCRNucleic Acids Research, 2002