High Performance Liquid Chromatography of Uroporphyrin Isomers
- 31 December 1982
- journal article
- research article
- Published by Informa UK Limited in Journal of Liquid Chromatography
- Vol. 6 (2), 383-394
- https://doi.org/10.1080/01483918308066898
Abstract
The separation of uroporphyrin I and III isomers by reversed-phase high performance liquid chromatography on ODS-Hypersil with ammonium acetate buffer-acetonitrile solvent systems is described. The effects of buffer concentration, pH, organic modifier proportion and different organic modifiers on the resolution are studied. The optimum conditions for the separation were 12–13% acetonitrile in 1M ammonium acetate buffer pH 5.10–5.20. The method also separated uroporphyrin I and III from the II isomers but the resolution of uroporphyrin III and IV isomers was not achieved.This publication has 8 references indexed in Scilit:
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