Tissue plasminogen activator (tPA) antigen in plasma: correlation with different tPA/inhibitor complexes
- 1 January 1998
- journal article
- Published by Taylor & Francis Ltd in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 58 (6), 475-484
- https://doi.org/10.1080/00365519850186274
Abstract
In many studies, tPA antigen has been a strong predictor of myocardial infarction. However, only a few percent of the total tPA antigen present in plasma samples taken at rest constitutes active tPA. The rest is enzymatically inactive and consists of a heterogeneous mixture of tPA in complex with inhibitors such as PAI-1, antiplasmin and C1 inhibitor. In the present study we developed specific two-site ELISA methods for determining the individual protease/inhibitor complexes constituting tPA antigen. We subsequently measured the concentrations of the different complexes in plasma samples taken from 30 healthy individuals. The results show that the concentration of the complex between tPA and PAI-1 in plasma correlated strongly with that of tPA antigen in plasma, as measured with a commercially available kit. Also the correlation between tPA/PAI-1 complex levels in plasma and the PAI-1 activity concentration was significant. However, no significant correlations were found between tPA antigen concentration and tPA/C1 inhibitor or tPA/antiplasmin concentrations in plasma.Keywords
This publication has 7 references indexed in Scilit:
- The Significance of Hypofibrinolysis for the Risk of Recurrence of Venous ThromboembolismThrombosis and Haemostasis, 1996
- Plasminogen activator inhibitor 1 in thrombotic diseaseCurrent Opinion in Hematology, 1996
- Impaired fibrinolysis and risk of thromboembolismProgress in Cardiovascular Diseases, 1991
- Purification of high and low molecular weight plasminogen activator inhibitor 1 from fibrosarcoma cell-line HT 1080 conditioned mediumBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1989
- Plasminogen activator in normal subjects after exercise and venous occlusion: t-PA circulates as complexes with C1-inhibitor and PAI-1Blood, 1987
- Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassaysAnalytical Biochemistry, 1984
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970