Preabsorptive Metabolism of Sodium Arsenate by Anaerobic Microbiota of Mouse Cecum Forms a Variety of Methylated and Thiolated Arsenicals
- 9 March 2011
- journal article
- other
- Published by American Chemical Society (ACS) in Chemical Research in Toxicology
- Vol. 24 (4), 475-477
- https://doi.org/10.1021/tx200040w
Abstract
The conventional scheme for arsenic methylation accounts for methylated oxyarsenical production but not for thioarsenical formation. Here, we report that in vitro anaerobic microbiota of mouse cecum converts arsenate into oxy- and thio- arsenicals. Besides methylarsonic acid (MMA(V)), arsenate was transformed into six unique metabolites: mono-, di-, and trithio-arsenic acid, monomethyldithio- and monomethyltrithio-arsonic acid, and dimethyldithioarsonic acid. Thioarsenicals were found in soluble and particulate fractions of reaction mixtures, suggesting interactions with anaerobic microbiota. Metabolism of ingested arsenate to oxy- and thio-arsenicals before absorption across the gastrointestinal barrier could affect bioavailability, systemic distribution, and resulting toxicity.Keywords
This publication has 19 references indexed in Scilit:
- Arsenic Metabolism by Human Gut Microbiota upon in Vitro Digestion of Contaminated SoilsEnvironmental Health Perspectives, 2010
- In Vitro Assays of Inorganic Arsenic MethylationCurrent Protocols in Toxicology, 2009
- ACUTE TOXICITY OF THIOARSENATES TO VIBRIO FISCHERIEnvironmental Toxicology and Chemistry, 2008
- Pentavalent Arsenic Can Bind to BiomoleculesAngewandte Chemie, 2007
- Metabolic differences between two dimethylthioarsenicals in ratsToxicology and Applied Pharmacology, 2007
- Chromatographic Separation and Identification of Products from the Reaction of Dimethylarsinic Acid with Hydrogen SulfideChemical Research in Toxicology, 2005
- A new metabolic pathway of arsenite: arsenic?glutathione complexes are substrates for human arsenic methyltransferase Cyt19Archives of Toxicology, 2004
- Endogenous production of hydrogen sulfide in mammalsAmino Acids, 2004
- In VitroMethylation of Inorganic Arsenic in Mouse Intestinal CecumToxicology and Applied Pharmacology, 1997
- In vitro metabolism of inorganic arsenic by the gastro‐intestinal microflora of the ratJournal of Applied Toxicology, 1981