Experimental Mapping of the Canine KCNJ2 and KCNJ12 Gene Structures and Functional Analysis of the Canine KIR2.2 ion Channel
Open Access
- 1 January 2012
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Physiology
- Vol. 3, 14377
- https://doi.org/10.3389/fphys.2012.00009
Abstract
For many model organisms traditionally in use for cardiac electrophysiological studies, characterization of ion channel genes is lacking. We focused here on two genes encoding the inward rectifier current, KCNJ2 and KCNJ12, in the dog heart. A combination of RT-PCR, 5′-RACE, and 3′-RACE demonstrated the status of KCNJ2 as a two exon gene. The complete open reading frame (ORF) was located on the second exon. One transcription initiation site was mapped. Four differential transcription termination sites were found downstream of two consensus polyadenylation signals. The canine KCNJ12 gene was found to consist of three exons, with its ORF located on the third exon. One transcription initiation and one termination site were found. No alternative splicing was observed in right ventricle or brain cortex. The gene structure of canine KCNJ2 and KCNJ12 was conserved amongst other vertebrates, while current GenBank gene annotation was determined as incomplete. In silico translation of KCN12 revealed a non-conserved glycine rich stretch located near the carboxy-terminus of the KIR2.2 protein. However, no differences were observed when comparing dog with human KIR2.2 protein upon ectopic expression in COS-7 or HEK293 cells with respect to subcellular localization or electrophysiological properties.This publication has 34 references indexed in Scilit:
- Mutations in Potassium Channel Kir2.6 Cause Susceptibility to Thyrotoxic Hypokalemic Periodic ParalysisCell, 2010
- Remodelling of cardiac repolarization: how homeostatic responses can lead to arrhythmogenesisCardiovascular Research, 2008
- The canine model with chronic, complete atrio-ventricular blockPharmacology & Therapeutics, 2008
- The molecular basis of chloroquine block of the inward rectifier Kir2.1 channelProceedings of the National Academy of Sciences of the United States of America, 2008
- Low‐affinity spermine block mediating outward currents through Kir2.1 and Kir2.2 inward rectifier potassium channelsThe Journal of Physiology, 2007
- Regional and tissue specific transcript signatures of ion channel genes in the non‐diseased human heartThe Journal of Physiology, 2007
- Genome sequence, comparative analysis and haplotype structure of the domestic dogNature, 2005
- A Novel Form of Short QT Syndrome (SQT3) Is Caused by a Mutation in the KCNJ2 GeneCirculation Research, 2005
- The consequences of disrupting cardiac inwardly rectifying K+ current (IK1) as revealed by the targeted deletion of the murine Kir2.1 and Kir2.2 genesThe Journal of Physiology, 2001
- The Human Inward Rectifying K+Channel Kir 2.2 (KCNJ12) Gene: Gene Structure, Assignment to Chromosome 17p11.1, and Identification of a Simple Tandem Repeat PolymorphismGenomics, 1997