Ratiometric Photoacoustic Molecular Imaging for Methylmercury Detection in Living Subjects
- 22 February 2017
- journal article
- research article
- Published by Wiley in Advanced Materials
- Vol. 29 (17)
- https://doi.org/10.1002/adma.201606129
Abstract
Photoacoustic molecular imaging is an emerging and promising diagnostic tool for heavy metal ions detection. Methylmercury (MeHg+) is one of the most potent neurotoxins, which damages the brain and nervous system of human beings through fish consumption. The development of a selective and sensitive method for MeHg+ detection is highly desirable. In this Communication, we develope a chemoselective photoacoustic sensor (LP‐hCy7) composed of the liposome (LP) and MeHg+‐responsive near‐infrared (NIR) cyanine dye (hCy7) for MeHg+ detection within living subjects, such as zebrafish and mouse. The as‐prepared LP‐hCy7 nanoprobe displays unique dual‐shift NIR absorbance peaks and produces a normalized turn‐on response after the reaction of MeHg+ and hCy7 through a mercury‐promoted cyclization reaction. The absorbance intensities of LP‐hCy7 nanoprobe at 690 and 860 nm are decreased and increased, respectively. The ratiometric photoacoustic signal (PA860/PA690) is noticeably increased in the presence of MeHg+. These findings not only provide a ratiometric photoacoustic molecular imaging probe for the detection of metal ions in vivo, but also provides a tool for spectroscopic photoacoustic molecular imaging.Keywords
Funding Information
- National Natural Science Foundation of China (51573096, 81601531, 81401465)
- China Postdoctoral Science Foundation (2016M600671)
- National Institutes of Health
- National Institute of Biomedical Imaging and Bioengineering
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