Characterization of cartilage metabolic response to static and dynamic stress using a mechanical explant test system
Open Access
- 1 January 1997
- journal article
- research article
- Published by Elsevier BV in Journal of Biomechanics
- Vol. 30 (1), 1-9
- https://doi.org/10.1016/s0021-9290(96)00117-0
Abstract
A new mechanical explant test system was used to study the metabolic response (via proteoglycan biosynthesis) of mature, weight-bearing canine articular cartilage subjected to static and dynamic compressive stresses. Stresses ranging from 0.5 to 24 MPa were applied sinusoidally at 1 Hz for intervals of 2-24 h. The explants were loaded in unconfined compression and compared to age-matched unloaded explants. Both static and dynamic compressive stress significantly decreased proteoglycan biosynthesis (range 25-85%) for all loading time intervals. The inhibition was proportional to the applied stress but was independent of loading time. After rehydration upon load removal, the measured water content of the loaded explants was not different from the unloaded explants for all test variables. Autoradiographic and electron microscopic analysis of loaded explants showed viable chondrocytes throughout the matrix. Our results suggest that the decreased metabolic response of cyclically loaded explants may be dominated by the static component (RMS) of the dynamic load. Furthermore, the observed decreased metabolism may be more representative of the in situ tissue response than that of unloaded explants, in which we found an increasing rate of metabolism for up to 6 days after explant removal.Keywords
This publication has 38 references indexed in Scilit:
- Local stimulation of proteoglycan synthesis in articular cartilage explants by dynamic compression in vitroJournal of Orthopaedic Research, 1992
- An Analytical Model of Joint ContactJournal of Biomechanical Engineering, 1990
- Influence of cyclic loading on the nutrition of articular cartilage.Annals Of The Rheumatic Diseases, 1990
- Bone and cartilage changes following experimental varus or valgus tibial angulationJournal of Orthopaedic Research, 1990
- Biochemical changes in articular cartilage after joint immobilization by casting or external fixationJournal of Orthopaedic Research, 1989
- Softening of Canine Articular Cartilage After Immobilization of the Knee JointPublished by Ovid Technologies (Wolters Kluwer Health) ,1986
- The Effect of Continuous Mechanical Pressure Upon the Turnover of Articular Cartilage Proteoglycans In vitroPublished by Ovid Technologies (Wolters Kluwer Health) ,1982
- Load-Bearing Mode of the Knee JointClinical Orthopaedics and Related Research, 1980
- Biphasic Creep and Stress Relaxation of Articular Cartilage in Compression: Theory and ExperimentsJournal of Biomechanical Engineering, 1980
- In vitro rabbit articular cartilage organ model I. Morphology and glycosaminoglycan metabolismArthritis & Rheumatism, 1974