Characterization of the necrosis determinant of the European genotype of pepino mosaic virus by site-specific mutagenesis of an infectious cDNA clone
- 9 November 2011
- journal article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 157 (2), 337-341
- https://doi.org/10.1007/s00705-011-1162-1
Abstract
Mild and necrotic isolates have been described for the European (EU) genotype of pepino mosaic virus (PepMV), an important pathogen of tomato worldwide. In this study, we produced various infectious cDNA clones of an EU isolate with point mutations introduced by site-directed mutagenesis. Our results showed that the genetic determinant responsible for necrosis induction on tomato and Datura inoxia was amino acid 67 of TGBp3. This amino acid residue also acts as necrosis determinant in PepMV isolates belonging to the Chilean 2 genotype. This demonstrates that a single point mutation plays a role in necrosis induction by PepMV, irrespective of genotype.Keywords
This publication has 29 references indexed in Scilit:
- Mixed Infections of Pepino Mosaic Virus Strains Modulate the Evolutionary Dynamics of this Emergent VirusJournal of Virology, 2009
- Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombinationEuropean Journal of Plant Pathology, 2007
- Creation of diversity in the animal virus world by inter-species and intra-species recombinations: lessons learned from poultry virusesVirus Genes, 2007
- Molecular characterization of two Pepino mosaic virus variants from imported tomato seed reveals high levels of sequence identity between Chilean and US isolatesVirus Genes, 2007
- New Polish Isolate of Pepino mosaic virus Highly Distinct from European Tomato, Peruvian, and US2 StrainsPlant Disease, 2006
- Effect of Pepino mosaic virus on the yield and quality of glasshouse‐grown tomatoes in the UKPlant Pathology, 2006
- Genetic Structure of the Population of Pepino mosaic virus Infecting Tomato Crops in SpainPhytopathology®, 2006
- Two unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European-like) US isolateArchiv für die gesamte Virusforschung, 2005
- First Report of Pepino mosaic virus in Canada and the United StatesPlant Disease, 2001
- First Report of Pepino Mosaic Virus on TomatoPlant Disease, 2000