Microfluidics for Mammalian Cell Chemotaxis
- 22 December 2011
- journal article
- review article
- Published by Springer Science and Business Media LLC in Annals of Biomedical Engineering
- Vol. 40 (6), 1316-1327
- https://doi.org/10.1007/s10439-011-0489-9
Abstract
The emerging field of micro-technology has opened up new possibilities for exploring cellular chemotaxis in real space and time, and at single cell resolution. Chemotactic cells sense and move in response to chemical gradients and play important roles in a number of physiological and pathological processes, including development, immune responses, and tumor cell invasions. Due to the size proximity of the microfluidic device to cells, microfluidic chemotaxis devices advance the traditional macro-scale chemotaxis assays in two major directions: one is to build well defined and stable chemical gradients at cellular length scales, and the other is to provide a platform for quantifying cellular responses at both cellular and molecular levels using advanced optical imaging systems. Here, we present a critical review on the designing principles, recent development, and potential capabilities of the microfluidic chemotaxis assay for solving problems that are of importance in the biomedical engineering field.Keywords
This publication has 90 references indexed in Scilit:
- Phosphorescent nanoparticles for quantitative measurements of oxygen profiles in vitro and in vivoBiomaterials, 2012
- Traction Forces of Neutrophils Migrating on Compliant SubstratesBiophysical Journal, 2011
- Controlled architectural and chemotactic studies of 3D cell migrationBiomaterials, 2011
- Hallmarks of Cancer: The Next GenerationCell, 2011
- Elucidating the Role of Matrix Stiffness in 3D Cell Migration and RemodelingBiophysical Journal, 2011
- Mechanisms of force generation and force transmission during interstitial leukocyte migrationEMBO Reports, 2010
- Dense type I collagen matrices that support cellular remodeling and microfabrication for studies of tumor angiogenesis and vasculogenesis in vitroBiomaterials, 2010
- Design, fabrication and implementation of a novel multi-parameter control microfluidic platform for three-dimensional cell culture and real-time imagingLab on a Chip, 2008
- Dissemination and growth of cancer cells in metastatic sitesNature Reviews Cancer, 2002
- Integrin-ligand binding properties govern cell migration speed through cell-substratum adhesivenessNature, 1997