Autotaxin promotes motility via G protein‐coupled phosphoinositide 3‐kinase γ in human melanoma cells
- 5 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 515 (1-3), 137-140
- https://doi.org/10.1016/s0014-5793(02)02457-2
Abstract
Autotaxin (ATX), an exo-nucleotide pyrophosphatase and phosphodiesterase, stimulates tumor cell motility at sub-nanomolar levels and augments invasiveness and angiogenesis. We investigated the role of G protein-coupled phosphoinositide 3-kinase γ (PI3Kγ) in ATX-mediated tumor cell motility stimulation. Pretreatment of human melanoma cell line A2058 with wortmannin or LY294002 inhibited ATX-induced motility. ATX increased the PI3K activity in p110γ, but not p85, immunoprecipitates. This effect was abrogated by PI3K inhibitors or inhibited by pertussis toxin. Furthermore, stimulation of tumor cell motility by ATX was inhibited by catalytically inactive form of PI3Kγ, strongly indicating the crucial role of PI3Kγ for ATX-mediated motility in human melanoma cellsKeywords
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