Selenium Promotes T-Cell Response to TCR-Stimulation and ConA, but Not PHA in Primary Porcine Splenocytes
Open Access
- 17 April 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (4), e35375
- https://doi.org/10.1371/journal.pone.0035375
Abstract
There is controversy in the literature over whether the selenium (Se) influences cellular immune responses, and the mechanisms possibly underlying these effects are unclear. In this study, the effects of Se on T-cell proliferation and IL-2 production were studied in primary porcine splenocytes. Splenocytes were treated with different mitogens in the presence of 0.5–4 µmol/L sodium selenite. Se significantly promoted T-cell receptor (TCR) or concanavalin A (ConA)-induced T-cell proliferation and IL-2 production but failed to regulate T-cell response to phytohemagglutinin (PHA). In addition, Se significantly increased the levels of cytosolic glutathione peroxidase (GPx1) and thioredoxin reductase 1 (TR1) mRNA, the activity of GPx1 and the concentration of reduced glutathione (GSH) in the unstimulated, or activated splenocytes. These results indicated that Se improved the redox status in all splenocytes, including unstimulated, TCR, ConA and PHA -stimulated, but only TCR and ConA-induced T-cell activation was affected by the redox status. N-acetylcysteine (NAC), a pharmacological antioxidant, increased T-cell proliferation and IL-2 production by TCR and ConA stimulated splenocytes but had no effect on the response to PHA in primary porcine splenocytes confirming that PHA-induced T-cell activation is insensitive to the redox status. We conclude that Se promotes GPx1 and TR1 expression and increases antioxidative capacity in porcine splenocytes, which enhances TCR or ConA -induced T-cell activation but not PHA-induced T-cell activation. The different susceptibilities to Se between the TCR, ConA and PHA -induced T-cell activation may help to explain the controversy in the literature over whether or not Se boosts immune responses.This publication has 42 references indexed in Scilit:
- The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and diseaseJournal of Leukocyte Biology, 2011
- Selenoproteins Are Essential for Proper Keratinocyte Function and Skin DevelopmentPLOS ONE, 2010
- Dietary Selenium Modulates Activation and Differentiation of CD4+ T Cells in Mice through a Mechanism Involving Cellular Free ThiolsJournal of Nutrition, 2010
- Regulation and function of selenoproteins in human diseaseBiochemical Journal, 2009
- Selenium status highly regulates selenoprotein mRNA levels for only a subset of the selenoproteins in the selenoproteomeBioscience Reports, 2009
- The piglet as a model for B cell and immune system developmentVeterinary Immunology and Immunopathology, 2009
- Transcript Analysis of the Selenoproteome Indicates That Dietary Selenium Requirements of Rats Based on Selenium-Regulated Selenoprotein mRNA Levels Are Uniformly Less Than Those Based on Glutathione Peroxidase ActivityJournal of Nutrition, 2009
- Selenoproteins Mediate T Cell Immunity through an Antioxidant MechanismJournal of Biological Chemistry, 2008
- From Selenium to Selenoproteins: Synthesis, Identity, and Their Role in Human HealthAntioxidants and Redox Signaling, 2007
- Effects of selenium and vitamin E on the immune responses of domestic animalsResearch in Veterinary Science, 1996