Genomes of Three Methylotrophs from a Single Niche Reveal the Genetic and Metabolic Divergence of the Methylophilaceae
- 1 August 2011
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 193 (15), 3757-3764
- https://doi.org/10.1128/jb.00404-11
Abstract
The genomes of three representatives of the family Methylophilaceae , Methylotenera mobilis JLW8, Methylotenera versatilis 301, and Methylovorus glucosetrophus SIP3-4, all isolated from a single study site, Lake Washington in Seattle, WA, were completely sequenced. These were compared to each other and to the previously published genomes of Methylobacillus flagellatus KT and an unclassified Methylophilales strain, HTCC2181. Comparative analysis revealed that the core genome of Methylophilaceae may be as small as approximately 600 genes, while the pangenome may be as large as approximately 6,000 genes. Significant divergence between the genomes in terms of both gene content and gene and protein conservation was uncovered, including the varied presence of certain genes involved in methylotrophy. Overall, our data demonstrate that metabolic potentials can vary significantly between different species of Methylophilaceae , including organisms inhabiting the very same environment. These data suggest that genetic divergence among the members of this family may be responsible for their specialized and nonredundant functions in C 1 cycling, which in turn suggests means for their successful coexistence in their specific ecological niches.Keywords
This publication has 37 references indexed in Scilit:
- Complete Genome Sequence of the Bacterium Methylovorus sp. Strain MP688, a High-Level Producer of Pyrroloquinolone QuinoneJournal of Bacteriology, 2011
- Expressed Genome of Methylobacillus flagellatus as Defined through Comprehensive Proteomics and New Insights into MethylotrophyJournal of Bacteriology, 2010
- Identification of Novel Methane-, Ethane-, and Propane-Oxidizing Bacteria at Marine Hydrocarbon Seeps by Stable Isotope ProbingApplied and Environmental Microbiology, 2010
- Microbial Community Changes in Response to Ethanol or Methanol Amendments for U(VI) ReductionApplied and Environmental Microbiology, 2010
- The Expanding World of Methylotrophic MetabolismAnnual Review of Microbiology, 2009
- Genomic islands from five strains of Burkholderia pseudomalleiBMC Genomics, 2008
- Attached Bacterial Populations Shared by Four Species of Aquatic AngiospermsApplied and Environmental Microbiology, 2008
- Identification of proteins involved in formaldehyde metabolism by Rhodobacter sphaeroidesMicrobiology, 2008
- Identification of a Fourth Formate Dehydrogenase in Methylobacterium extorquens AM1 and Confirmation of the Essential Role of Formate Oxidation in MethylotrophyJournal of Bacteriology, 2007
- Genome of Methylobacillus flagellatus , Molecular Basis for Obligate Methylotrophy, and Polyphyletic Origin of MethylotrophyJournal of Bacteriology, 2007