Elements regulating angiogenesis and correlative microvessel density in benign hyperplastic and malignant prostate tissue
- 1 June 2003
- journal article
- research article
- Published by Springer Science and Business Media LLC in Prostate Cancer and Prostatic Diseases
- Vol. 6 (2), 131-137
- https://doi.org/10.1038/sj.pcan.4500637
Abstract
Purpose: To quantify the ex vivo production of proangiogenic proteins (vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), urokinase-type plasminogen activator (u-PA), tissue-type plasminogen activator (tPA)) and angiogenesis inhibitors (plasminogen activator inhibitor type-1 (PAI-1) and angiostatin) from epithelial and stromal components of primary prostate cancer (CaP) and benign prostatic hyperplasia (BPH) cultures. To perform microvessel density (MVD) counts on sections of BPH and CaP from the same prostatectomy specimens. Scope: Angiogenic cytokine expression was measured by immunoassays and in vitro angiostatin generating capacities assessed using immunoblotting. CaP and BPH tissue was immunostained using factor VIII antibody to determine MVD. Conclusions: Elements regulating angiogenesis are present in both primary cultures of CaP and BPH, suggesting that angiogenic ability is well established in the absence of carcinoma.Keywords
This publication has 21 references indexed in Scilit:
- Relationship between prostate specific antigen density, microvessel density and prostatic volume in benign prostatic hyperplasia and advanced prostatic carcinomaInternational Urology and Nephrology, 1998
- Vascular Endothelial Growth Factor (VEGF) Expression in Human Prostate Cancer: In Situ and in Vitro Expression of VEGF by Human Prostate Cancer CellsJournal of Urology, 1997
- Vascular Endothelial Growth Factor (VEGF) Expression in Prostate Cancer and Benign Prostatic HyperplasiaJournal of Urology, 1997
- Angiogenesis: A Paradigm for Balanced Extracellular Proteolysis during Cell Migration and MorphogenesisEnzyme and Protein, 1996
- Urokinase-Type Plasminogen Activator Expression in Human Prostate CarcinomasThe American Journal of the Medical Sciences, 1996
- Angiostatin induces and sustains dormancy of human primary tumors in miceNature Medicine, 1996
- Antiangiogenic therapy of transgenic mice impairs de novo tumor growth.Proceedings of the National Academy of Sciences, 1996
- Potent synergism between vascular endothelial growth factor and basic fibroblast growth factor in the induction of angiogenesis in vitroBiochemical and Biophysical Research Communications, 1992
- Angiogenesis.Journal of Biological Chemistry, 1992
- Induction of angiogenesis during the transition from hyperplasia to neoplasiaNature, 1989