Comparison of MY09/11 consensus PCR and type‐specific PCRs in the detection of oncogenic HPV types
Open Access
- 2 July 2007
- journal article
- research article
- Published by Wiley in Journal of Cellular and Molecular Medicine
- Vol. 11 (4), 881-891
- https://doi.org/10.1111/j.1582-4934.2007.00073.x
Abstract
The causal relationship between persistent infection with high-risk HPV and cervical cancer has resulted in the development of HPV DNA detection systems. The widely used MY09/11 consensus PCR targets a 450bp conserved sequence in the HPV L1 gene, and can therefore amplify a broad spectrum of HPV types. However, limitations of these consensus primers are evident, particularly in regard to the variability in detection sensitivity among different HPV types. This study compared MY09/11 PCR with type-specific PCRs in the detection of oncogenic HPV types. The study population comprised 15, 774 patients. Consensus PCR failed to detect 522 (10.9%) HPV infections indicated by type-specific PCRs. A significant correlation between failure of consensus PCR and HPV type was found. HPV types 51, 68 and 45 were missed most frequently. The clinical relevance of the HPV infections missed by MY09/11 PCR was reflected in the fraction of cases with cytological abnormalities and in follow-up, showing 104 (25.4%) CIN2+ cases. The MY09/11 false negativity could be the result of poor sensitivity, mismatch of MY09/11 primers or disruption of L1 target by HPV integration or DNA degradation. Furthermore, MY09/11 PCR lacked specificity for oncogenic HPVs. Diagnostic accuracy of the PCR systems, in terms of sensitivity (MY09/11 PCR: 87.9%; type-specific PCRs: 98.3%) and specificity (MY09/11 PCR: 38.7%; type-specific PCRs: 76.14%), and predictive values for histologically confirmed CIN2+, suggest that type-specific PCRs could be used in a clinical setting as a reliable screening tool.Keywords
This publication has 37 references indexed in Scilit:
- The Expanded Use of HPV Testing in Gynecologic Practice per ASCCP-Guided Management Requires the Use of Well-Validated AssaysAmerican Journal of Clinical Pathology, 2007
- Distribution and viral load of eight oncogenic types of human papillomavirus (HPV) and HPV 16 integration status in cervical intraepithelial neoplasia and carcinomaLaboratory Investigation, 2007
- Improved endocervical sampling and HPV viral load detection by Cervex-Brush CombiCytopathology, 2006
- Clinical utility of HPV–DNA detection: Triage of minor cervical lesions, follow-up of women treated for high-grade CIN: An update of pooled evidenceGynecologic Oncology, 2005
- Molecular diagnosis of human papillomavirus (HPV) infectionsJournal of Clinical Virology, 2005
- Virologic Versus Cytologic Triage of Women With Equivocal Pap Smears: A Meta-analysis of the Accuracy To Detect High-Grade Intraepithelial NeoplasiaJNCI Journal of the National Cancer Institute, 2004
- Real-Time PCR-Based System for Simultaneous Quantification of Human Papillomavirus Types Associated with High Risk of Cervical CancerJournal of Clinical Microbiology, 2003
- Epidemiologic Classification of Human Papillomavirus Types Associated with Cervical CancerNew England Journal of Medicine, 2003
- Human papillomavirus types in invasive cervical cancer worldwide: a meta-analysisBritish Journal of Cancer, 2003
- HPV testing in primary screening of older womenBritish Journal of Cancer, 1999