Second harmonic imaging of plants tissues and cell implosion using two‐photon process in ZnO nanoparticles
- 2 November 2011
- journal article
- Published by Wiley in Journal of Biophotonics
- Vol. 5 (3), 283-291
- https://doi.org/10.1002/jbio.201100076
Abstract
The optical properties of colloidal ZnO nanoparticle (NP) solutions, with size ranging from several nm to around 200 nm, have been tailored to have high optical nonlinearity for bioimaging with no auto‐fluorescence above 750 nm and minimal auto‐fluorescence below 750 nm. The high second harmonic conversion efficiency enables selective tissue imaging and cell tracking using tunable near‐infrared femtosecond laser source ranging from 750‐980 nm. For laser energies exceeding the two‐photon energy of the bandgap of ZnO (half of 3.34 eV), the SHG signal greatly decreases and the two‐photon emission becomes the dominant signal. The heat generated due to two‐photon absorption within the ZnO NPs enable selective cell or localized tissue destruction using excitation wavelength ranging from 710–750 nm.Keywords
This publication has 29 references indexed in Scilit:
- Second harmonic generating (SHG) nanoprobes for in vivo imagingProceedings of the National Academy of Sciences, 2010
- Nanoparticles for biomedical imagingExpert Opinion on Drug Delivery, 2009
- Preparation of ZnO nanoparticles for bio‐imaging applicationsPhysica Status Solidi (b), 2009
- Quantum dots and nanoparticles for photodynamic and radiation therapies of cancerAdvanced Drug Delivery Reviews, 2008
- Reflection across plant cell boundaries in confocal laser scanning microscopyJournal of Microscopy, 2008
- Zinc Oxide Nanocrystals for Nonresonant Nonlinear Optical Microscopy in Biology and MedicineThe Journal of Physical Chemistry C, 2008
- Preferential killing of cancer cells and activated human T cells using ZnO nanoparticlesNanotechnology, 2008
- Selective toxicity of zinc oxide nanoparticles to prokaryotic and eukaryotic systemsApplied Physics Letters, 2007
- Principles of two-photon excitation fluorescence microscopy and other nonlinear imaging approachesAdvanced Drug Delivery Reviews, 2006
- Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolutionProceedings of the National Academy of Sciences of the United States of America, 2005