Effect of lactoferrin on Enteroaggregative E. coli (EAEC)This paper is one of a selection of papers published in this Special Issue, entitled 7th International Conference on Lactoferrin: Structure, Function, and Applications, and has undergone the Journal’s usual peer review process.
- 1 June 2006
- journal article
- research article
- Published by Canadian Science Publishing in Biochemistry and Cell Biology
- Vol. 84 (3), 369-376
- https://doi.org/10.1139/o06-053
Abstract
We previously demonstrated that lactoferrin inhibits adherence of enteropathogenic Escherichia coli to HEp-2 cells and decreases invasiveness of Shigella flexneri in HeLa cells by disruption of the type III secretory system (TTSS) of both enteropathogens. To determine whether these effects were specific to the TTSS, we assessed the activity of bovine lactoferrin on enteroaggregative E. coli (EAEC), enteropathogens whose virulence is not TTSS dependent. Bovine lactoferrin at a concentration of 1.0 and 0.1 mg/mL inhibited EAEC growth. Saturation with iron reversed the bacteriostatic effect. Lactoferrin under nonbacteriostatic conditions decreased EAEC adherence to HEp-2 cells as evaluated by microscopy and CFUs; this effect was not iron dependent. Lactoferrin inhibited EAEC biofilm formation and increased autoagglutination. Lactoferrin blocks EAEC adherence by inducing release and degradation of aggregative adherence fimbria, a key element of EAEC pathogenesis. We hypothesized that lactoferrin binding to lipid A of lipopolysaccharide disrupts the virulence proteins anchored to the bacterial outermembrane. These data suggest that the effect of lactoferrin on surface proteins is not restricted to organisms having a TTSS.Keywords
This publication has 32 references indexed in Scilit:
- Prevalence of diarrheagenic Escherichia coli in acute childhood enteritis: A prospective controlled studyThe Journal of Pediatrics, 2005
- Improved Prediction of Signal Peptides: SignalP 3.0Journal of Molecular Biology, 2004
- Lactoferrin—a multifunctional protein with antimicrobial propertiesMolecular Immunology, 2003
- How many child deaths can we prevent this year?The Lancet, 2003
- Human Lactoferrin Impairs Virulence ofShigella flexneriThe Journal of Infectious Diseases, 2003
- Lactoferrin Protects Rabbits fromShigella flexneri-Induced Inflammatory EnteritisInfection and Immunity, 2002
- The Staphylococcus aureus Map protein is an immunomodulator that interferes with T cell–mediated responsesJCI Insight, 2002
- Binding of lactoferrin and free secretory component to enterotoxigenic Escherichia coliFEMS Microbiology Letters, 2001
- Resistance to Lyme disease in decorin-deficient miceJCI Insight, 2001
- Biophysical Characterization of Lipopolysaccharide and Lipid A Inactivation by LactoferrinBiological Chemistry, 2001