Mapping and analysis of phosphorylation sites: a quick guide for cell biologists
Open Access
- 1 March 2013
- journal article
- review article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 24 (5), 535-542
- https://doi.org/10.1091/mbc.e12-09-0677
Abstract
A mechanistic understanding of signaling networks requires identification and analysis of phosphorylation sites. Mass spectrometry offers a rapid and highly sensitive approach to mapping phosphorylation sites. However, mass spectrometry has significant limitations that must be considered when planning to carry out phosphorylation-site mapping. Here we provide an overview of key information that should be taken into consideration before beginning phosphorylation-site analysis, as well as a step-by-step guide for carrying out successful experiments.Keywords
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