Propofol suppresses proliferation and invasion of glioma cells by upregulating microRNA-218 expression
Open Access
- 1 July 2015
- journal article
- Published by Spandidos Publications in Molecular Medicine Reports
- Vol. 12 (4), 4815-4820
- https://doi.org/10.3892/mmr.2015.4014
Abstract
Propofol (2,6-diisopropylphenol) is a commonly used intravenous anesthetic agent. The present study aimed to assess the effect of propofol on the proliferation and invasion of human glioma cells, and to determine the potential underlying molecular mechanisms. The effects of propofol on U373 glioblastoma cell proliferation, apoptosis and invasion were detected by an MTT assay, caspase-3 activity measurement and a Matrigel™ invasion assay, respectively. MicroRNA (miR)-218 expression and matrix metalloproteinase (MMP)-2 protein expression levels were analyzed by quantitative polymerase chain reaction and western blot analysis, respectively. In addition, miR-218 precursor was transfected into the cells to assess whether overexpression of miR-218 could affect MMP-2 expression. Anti-miR-218 was transfected into the cells to evaluate the role of miR-218 in the effects of propofol on the biological behavior of glioma cells. The results of the present study demonstrated that propofol significantly increased the expression levels of miR-218, inhibited U373 cell proliferation and invasion, and facilitated apoptosis. In addition, treatment with propofol efficiently reduced MMP-2 protein expression levels, and overexpression of miR-218 also decreased MMP-2 protein expression levels. Whereas, neutralization of miR-218 using the anti-miR-218 antibody reversed the effects of propofol on the biological behavior of U373 cells, and on the inhibition of MMP-2 protein expression. In conclusion, propofol may effectively suppress proliferation and invasion, and induce the apoptosis of glioma cells, at least partially through upregulation of miR-218 expression.Keywords
This publication has 37 references indexed in Scilit:
- MiRNA-329 targeting E2F1 inhibits cell proliferation in glioma cellsJournal of Translational Medicine, 2013
- Tumor suppressive microRNA-218 inhibits cancer cell migration and invasion by targeting focal adhesion pathways in cervical squamous cell carcinomaInternational Journal of Oncology, 2013
- MicroRNA-195 Inhibits the Proliferation of Human Glioma Cells by Directly Targeting Cyclin D1 and Cyclin E1PLOS ONE, 2013
- MiR-218 sensitizes glioma cells to apoptosis and inhibits tumorigenicity by regulating ECOP-mediated suppression of NF-κB activityNeuro-Oncology, 2012
- miRNA-218 contributes to the regulation of D-glucuronyl C5-epimerase expression in normal and tumor breast tissuesEpigenetics, 2012
- MMP-2 siRNA Inhibits Radiation-Enhanced Invasiveness in Glioma CellsPLOS ONE, 2011
- An Integrated Genomic Analysis of Human Glioblastoma MultiformeScience, 2008
- Radiotherapy plus Concomitant and Adjuvant Temozolomide for GlioblastomaNew England Journal of Medicine, 2005
- Principles of MicroRNA–Target RecognitionPLoS Biology, 2005
- New functions for the matrix metalloproteinases in cancer progressionNature Reviews Cancer, 2002