Serum Cholinesterase Inhibition in Relation to Paraoxonase-1 (PON1) Status among Organophosphate-Exposed Agricultural Pesticide Handlers
- 1 September 2009
- journal article
- Published by Environmental Health Perspectives in Environmental Health Perspectives
- Vol. 117 (9), 1402-1408
- https://doi.org/10.1289/ehp.0900682
Abstract
Animal studies have demonstrated that low paraoxonase-1 (PON1) status (i.e., low catalytic efficiency and/or low plasma PON1 activity) is associated with neurotoxic effects after exposure to several organophosphate (OP) insecticides. However, few human studies have investigated associations between PON1 status and intermediate end points, such as serum cholinesterase [butyrylcholinesterase (BuChE)] inhibition, among OP-exposed individuals. We evaluated the relation between plasma PON1 status and BuChE inhibition among OP-exposed agricultural pesticide handlers. Agricultural pesticide handlers in Washington State were recruited during the 2006 and 2007 spray seasons when they were seen for follow-up ChE testing by collaborating medical providers as part of a statewide monitoring program. Blood samples were collected from 163 participants and tested for PON1 status based on plasma PON1 activity [arylesterase (AREase)] and PON1 Q192R genotype. We evaluated percent change in BuChE activity from baseline level in relation to PON1 status. We observed significantly greater BuChE inhibition among QQ homozygotes relative to RR homozygotes (p = 0.036). Lower levels of plasma PON1 activity were significantly associated with greater BuChE inhibition (p = 0.004). These associations remained after adjustment for year, days since baseline test, age, and OP exposure in the last 30 days. We found that both low PON1 catalytic efficiency (i.e., the Q192 alloform) and low plasma PON1 activity were associated with BuChE inhibition among OP-exposed agricultural pesticide handlers. Corroborative findings from future studies with prospective collection of blood samples for PON1 testing, more sensitive markers of OP-related effects, and larger sample sizes are needed.Keywords
This publication has 38 references indexed in Scilit:
- Paraoxonase 1 (PON1) status and substrate hydrolysisToxicology and Applied Pharmacology, 2009
- PON1Q192R genetic polymorphism modifies organophosphorous pesticide effects on semen quality and DNA integrity in agricultural workers from southern MexicoToxicology and Applied Pharmacology, 2008
- Organophosphate Pesticide Exposure and Neurobehavioral Performance in Agricultural and Nonagricultural Hispanic WorkersEnvironmental Health Perspectives, 2006
- Risk of Brain Tumors in Children and Susceptibility to Organophosphorus Insecticides: The Potential Role of Paraoxonase (PON1)Environmental Health Perspectives, 2005
- Blood Acylpeptide Hydrolase Activity Is a Sensitive Marker for Exposure to Some Organophosphate ToxicantsToxicological Sciences, 2005
- Association of Pesticide Exposure with Neurologic Dysfunction and DiseaseEnvironmental Health Perspectives, 2004
- Regulation of Serum Paraoxonase Activity by Genetic, Nutritional, and Lifestyle Factors in the General PopulationClinical Chemistry, 2003
- Paraoxonase and susceptibility to organophosphorus poisoning in farmers dipping sheepPharmacogenetics, 2003
- SIMULATED DERMAL CONTAMINATION WITH CAPILLARY SAMPLES AND FIELD CHOLINESTERASE BIOMONITORINCJournal of Toxicology and Environmental Health, 1997
- Human and rabbit paraoxonases: Purification, cloning, sequencing, mapping and role of polymorphism in organophosphate detoxificationChemico-Biological Interactions, 1993