A Novel Plasmid-Encoded Serotype Conversion Mechanism through Addition of Phosphoethanolamine to the O-Antigen of Shigella flexneri
Open Access
- 26 September 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (9), e46095
- https://doi.org/10.1371/journal.pone.0046095
Abstract
Shigella flexneri is the major pathogen causing bacillary dysentery in developing countries. S. flexneri is divided into at least 16 serotypes based on the combination of antigenic determinants present in the O-antigen. All the serotypes (except for serotype 6) share a basic O-unit containing one N-acetyl-d-glucosamine and three l-rhamnose residues, whereas differences between the serotypes are conferred by phage-encoded glucosylation and/or O-acetylation. Serotype Xv is a newly emerged and the most prevalent serotype in China, which can agglutinate with both MASF IV-1 and 7,8 monoclonal antibodies. The factor responsible for the presence of MASF IV-1 (E1037) epitope has not yet been identified. In this study, we analyzed the LPS structure of serotype Xv strains and found that the MASF IV-1 positive phenotype depends on an O-antigen modification with a phosphoethanolamine (PEtN) group attached at position 3 of one of the rhamnose residues. A plasmid carried gene, lpt-O (LPS phosphoethanolamine transferase for O–antigen), mediates the addition of PEtN for serotype Xv and other MASF IV-1 positive strains. These findings reveal a novel serotype conversion mechanism in S. flexneri and show the necessity of further extension of the serotype classification scheme recognizing the MASF IV-1 positive strains as distinctive subtypes.This publication has 39 references indexed in Scilit:
- Characterization of Unique Modification of Flagellar Rod Protein FlgG by Campylobacter jejuni Lipid A Phosphoethanolamine Transferase, Linking Bacterial Locomotion and Antimicrobial Peptide ResistanceOnline Journal of Public Health Informatics, 2012
- Genesis of a novel Shigella flexneri serotype by sequential infection of serotype-converting bacteriophages SfX and SfIBMC Microbiology, 2011
- Emergence of a Novel Shigella flexneri Serotype 4s Strain That Evolved from a Serotype X Variant in ChinaJournal of Clinical Microbiology, 2011
- Emergence of a New Multidrug-Resistant Serotype X Variant in an Epidemic Clone of Shigella flexneriJournal of Clinical Microbiology, 2010
- Functional Characterization of Lpt3 and Lpt6, the Inner-Core Lipooligosaccharide Phosphoethanolamine Transferases from Neisseria meningitidisJournal of Bacteriology, 2010
- Structural and Genetic Basis for the Serological Differentiation of Pasteurella multocida Heddleston Serotypes 2 and 5Journal of Bacteriology, 2009
- A Novel Glucosyltransferase Involved in O-Antigen Modification of Shigella flexneri Serotype 1cJournal of Bacteriology, 2009
- A Phosphoethanolamine Transferase Specific for the Outer 3-Deoxy-D-manno-octulosonic Acid Residue of Escherichia coli Lipopolysaccharide: IDENTIFICATION OF THE eptB GENE AND Ca2+ HYPERSENSITIVITY OF AN eptB DELETION MUTANTOnline Journal of Public Health Informatics, 2005
- Identification and functional analysis ofSalmonella entericaserovar Typhimurium PmrA-regulated genesFEMS Immunology & Medical Microbiology, 2005
- The Chromosome of Shigella flexneri Bacteriophage Sf6: Complete Nucleotide Sequence, Genetic Mosaicism, and DNA PackagingJournal of Molecular Biology, 2004