Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells
- 2 October 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (25), 5869-5874
- https://doi.org/10.1016/j.febslet.2006.09.053
Abstract
Vascular endothelial growth factor (VEGF), a potent mitogen for vascular endothelial cells, has been suggested as a modulator that is involved in neurogenesis as well as angiogenesis. Here, we directly examined the effect of VEGF on neuroectodermal differentiation using human embryonic stem cells (hESCs). VEGF treatment upregulated the expression of neuroectodermal genes (Sox1 and Nestin) during germ layer formation in embryoid bodies (EBs) and efficiently increased the number of neural rosettes expressing both Pax6 and Nestin. The neural progenitors generated from VEGF-treated EBs further differentiated into cells that showed a similar pattern of gene expression observed in the development of dopaminergic neurons upon terminal differentiation. These results support the neurogenic effect of VEGF on hESC differentiationThis publication has 35 references indexed in Scilit:
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