Monitoring of microbial hydrocarbon remediation in the soil
Open Access
- 6 July 2011
- journal article
- review article
- Published by Springer Science and Business Media LLC in 3 Biotech
- Vol. 1 (3), 117-138
- https://doi.org/10.1007/s13205-011-0014-8
Abstract
Bioremediation of hydrocarbon pollutants is advantageous owing to the cost-effectiveness of the technology and the ubiquity of hydrocarbon-degrading microorganisms in the soil. Soil microbial diversity is affected by hydrocarbon perturbation, thus selective enrichment of hydrocarbon utilizers occurs. Hydrocarbons interact with the soil matrix and soil microorganisms determining the fate of the contaminants relative to their chemical nature and microbial degradative capabilities, respectively. Provided the polluted soil has requisite values for environmental factors that influence microbial activities and there are no inhibitors of microbial metabolism, there is a good chance that there will be a viable and active population of hydrocarbon-utilizing microorganisms in the soil. Microbial methods for monitoring bioremediation of hydrocarbons include chemical, biochemical and microbiological molecular indices that measure rates of microbial activities to show that in the end the target goal of pollutant reduction to a safe and permissible level has been achieved. Enumeration and characterization of hydrocarbon degraders, use of micro titer plate-based most probable number technique, community level physiological profiling, phospholipid fatty acid analysis, 16S rRNA- and other nucleic acid-based molecular fingerprinting techniques, metagenomics, microarray analysis, respirometry and gas chromatography are some of the methods employed in bio-monitoring of hydrocarbon remediation as presented in this review.This publication has 138 references indexed in Scilit:
- Metagenomic Analyses: Past and Future TrendsApplied and Environmental Microbiology, 2011
- Accessing the Soil Metagenome for Studies of Microbial DiversityApplied and Environmental Microbiology, 2011
- Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for BioremediationMicrobiology and Molecular Biology Reviews, 2010
- Bacterial Diversity in a Mine Water Treatment PlantApplied and Environmental Microbiology, 2009
- Analysis of Bacterial Communities in Soil by Use of Denaturing Gradient Gel Electrophoresis and Clone Libraries, as Influenced by Different Reverse PrimersApplied and Environmental Microbiology, 2008
- Phylogenetic Diversity of Gram-Positive Bacteria Cultured from Marine SedimentsApplied and Environmental Microbiology, 2007
- The Microbial Community Structure in Petroleum-Contaminated Sediments Corresponds to Geophysical SignaturesApplied and Environmental Microbiology, 2007
- Microbial Population Dynamics Associated with Crude-Oil Biodegradation in Diverse SoilsApplied and Environmental Microbiology, 2006
- Structure and functional analyses of bacterial communities changes in microbial mats following petroleum exposureOphelia, 2004
- Bioremediation of oil-contaminated soil: microbiological methods for feasibility assessment and field evaluationJournal of Microbiological Methods, 1998