Histone chaperones regulate histone exchange during transcription
- 4 October 2007
- journal article
- Published by Springer Science and Business Media LLC in The EMBO Journal
- Vol. 26 (21), 4467-4474
- https://doi.org/10.1038/sj.emboj.7601870
Abstract
Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre‐existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.This publication has 45 references indexed in Scilit:
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