The John Charnley Award: An Accurate and Sensitive Method to Separate, Display, and Characterize Wear Debris: Part 1: Polyethylene Particles
- 1 February 2012
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Clinical Orthopaedics and Related Research
- Vol. 470 (2), 329-338
- https://doi.org/10.1007/s11999-011-2057-x
Abstract
Numerous studies indicate highly crosslinked polyethylenes reduce the wear debris volume generated by hip arthroplasty acetabular liners. This, in turns, requires new methods to isolate and characterize them. We describe a method for extracting polyethylene wear particles from bovine serum typically used in wear tests and for characterizing their size, distribution, and morphology. Serum proteins were completely digested using an optimized enzymatic digestion method that prevented the loss of the smallest particles and minimized their clumping. Density-gradient ultracentrifugation was designed to remove contaminants and recover the particles without filtration, depositing them directly onto a silicon wafer. This provided uniform distribution of the particles and high contrast against the background, facilitating accurate, automated, morphometric image analysis. The accuracy and precision of the new protocol were assessed by recovering and characterizing particles from wear tests of three types of polyethylene acetabular cups (no crosslinking and 5 Mrads and 7.5 Mrads of gamma irradiation crosslinking). The new method demonstrated important differences in the particle size distributions and morphologic parameters among the three types of polyethylene that could not be detected using prior isolation methods. The new protocol overcomes a number of limitations, such as loss of nanometer-sized particles and artifactual clumping, among others. The analysis of polyethylene wear particles produced in joint simulator wear tests of prosthetic joints is a key tool to identify the wear mechanisms that produce the particles and predict and evaluate their effects on periprosthetic tissues.Keywords
This publication has 49 references indexed in Scilit:
- Comparison of periprosthetic tissue digestion methods for ultra‐high molecular weight polyethylene wear debris extractionJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2009
- Cross-linked Compared with Historical Polyethylene in THA: An 8-year Clinical StudyClinical Orthopaedics and Related Research, 2008
- Isolation and characterization of UHMWPE wear particles down to ten nanometers in size from in vitro hip and knee joint simulatorsJournal of Biomedical Materials Research Part A, 2006
- Periprosthetic osteolysis: an immunologistʼs updateCurrent Opinion in Rheumatology, 2006
- Crosslinked polyethylene compared to conventional polyethylene in total hip replacement: Pre-clinical evaluation, in-vitro testing and prospective clinical follow-up studyActa Orthopaedica, 2006
- Wear, Debris, and Biologic Activity of Cross-linked Polyethylene in the KneeClinical Orthopaedics and Related Research, 2004
- Particle size and morphology of UHMWPE wear debris in failed total knee arthroplasties—a comparison between mobile bearing and fixed bearing kneesJournal of Orthopaedic Research, 2002
- Standardized analysis of UHMWPE wear particles from failed total joint arthroplastiesJournal of Biomedical Materials Research, 2002
- Shapes and dimensional characteristics of polyethylene wear particles generatedin vivo by total knee replacements compared to total hip replacementsJournal of Biomedical Materials Research, 1997
- Stimulation of Proteinase K Action by Denaturing Agents: Application to the Isolation of Nucleic Acids and the Degradation of ‘Masked’ ProteinsJBIC Journal of Biological Inorganic Chemistry, 1975