Application of the Papanicolaou Stain to Paraffin Sections
- 1 January 1956
- journal article
- research article
- Published by Informa UK Limited in Stain Technology
- Vol. 31 (5), 223-225
- https://doi.org/10.3109/10520295609113809
Abstract
Routine paraffin sections from tissues fixed either in aqueous formalin, 80% alcohol (with or without 1% trichloracetic acid added), Carnoy's alcohol-chloroform-acetic (6:3:1) and Bouin's fixative were stained as follows: Harris' hematoxylin, 6 min; running water, 2-3 min; ascending grades of alcohol to 95%; orange G, 0.5% and phosphotungstic acid, 0.015% in 95% alcohol, 5 min; 95% alcohol, 2 changes; Papanicolaou's EA36, 2.5 min; dehydration, clearing, and covering in Permount. The results show morphology better than hematoxylin and eosin and the technic is recommended particularly for keratin, which always stains bright orange.Keywords
This publication has 3 references indexed in Scilit:
- DIAGNOSIS OF UTERINE CANCER BY THE VAGINAL SMEARThe American Journal of the Medical Sciences, 1943
- A New Procedure for Staining Vaginal SmearsScience, 1942
- The sexual cycle in the human female as revealed by vaginal smearsJournal of Anatomy, 1933