PGC-1α plays a functional role in exercise-induced mitochondrial biogenesis and angiogenesis but not fiber-type transformation in mouse skeletal muscle
- 1 March 2010
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 298 (3), C572-C579
- https://doi.org/10.1152/ajpcell.00481.2009
Abstract
Endurance exercise stimulates peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α) expression in skeletal muscle, and forced expression of PGC-1α changes muscle metabolism and exercise capacity in mice. However, it is unclear if PGC-1α is indispensible for endurance exercise-induced metabolic and contractile adaptations in skeletal muscle. In this study, we showed that endurance exercise-induced expression of mitochondrial enzymes (cytochrome oxidase IV and cytochrome c) and increases of platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31)-positive endothelial cells in skeletal muscle, but not IIb-to-IIa fiber-type transformation, were significantly attenuated in muscle-specific Pgc-1α knockout mice. Interestingly, voluntary running effectively restored the compromised mitochondrial integrity and superoxide dismutase 2 (SOD2) protein expression in skeletal muscle in Pgc-1α knockout mice. Thus, PGC-1α plays a functional role in endurance exercise-induced mitochondrial biogenesis and angiogenesis, but not IIb-to-IIa fiber-type transformation in mouse skeletal muscle, and the improvement of mitochondrial morphology and antioxidant defense in response to endurance exercise may occur independently of PGC-1α function. We conclude that PGC-1α is required for complete skeletal muscle adaptations induced by endurance exercise in mice.Keywords
This publication has 55 references indexed in Scilit:
- p38γ Mitogen-Activated Protein Kinase Is a Key Regulator in Skeletal Muscle Metabolic Adaptation in MicePLOS ONE, 2009
- AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activityNature, 2009
- AMPK and PPARδ Agonists Are Exercise MimeticsCell, 2008
- Functional interaction of regulatory factors with the Pgc-1α promoter in response to exercise by in vivo imagingAmerican Journal of Physiology-Cell Physiology, 2008
- Protein Kinase D1 Stimulates MEF2 Activity in Skeletal Muscle and Enhances Muscle PerformanceMolecular and Cellular Biology, 2008
- Abnormal glucose homeostasis in skeletal muscle–specific PGC-1α knockout mice reveals skeletal muscle–pancreatic β cell crosstalkJCI Insight, 2007
- Histone deacetylase degradation andMEF2 activation promote the formation of slow-twitch myofibersJCI Insight, 2007
- PGC-1α Deficiency Causes Multi-System Energy Metabolic Derangements: Muscle Dysfunction, Abnormal Weight Control and Hepatic SteatosisPLoS Biology, 2005
- Regulation of Muscle Fiber Type and Running Endurance by PPARδPLoS Biology, 2004
- Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibresNature, 2002