OsRAD51D promotes homologous pairing and recombination by preventing nonhomologous interactions in rice meiosis
- 1 August 2020
- journal article
- research article
- Published by Wiley in New Phytologist
- Vol. 227 (3), 824-839
- https://doi.org/10.1111/nph.16595
Abstract
Homologous recombination is carefully orchestrated to maintain genome integrity. RAD51D has been previously shown to be essential for double-strand break repair in mammalian somatic cells. However, the function of RAD51D during meiosis is largely unknown. Here, through detailed analyses of Osrad51d single and double mutants, we pinpoint the specific function of OsRAD51D in coordinating homologous pairing and recombination by preventing nonhomologous interactions during meiosis. OsRAD51D is associated with telomeres in both meiocytes and somatic cells. Loss of OsRAD51D leads to significant induction of nonhomologous pairing and chromosome entanglements, suggesting its role in suppressing nonhomologous interactions. The failed localization of OsRAD51 and OsDMC1 in Osrad51d, together with the genetic analysis of Osrad51d Osdmc1a Osdmc1b, indicates that OsRAD51D acts at a very early stage of homologous recombination. Observations from the Osrad51d pair1 and Osrad51d ku70 double mutants further demonstrate that nonhomologous interactions require double-strand break formation but do not depend on the KU70-mediated repair pathway. Moreover, the interplay between OsRAD51D and OsRAD51C indicates both conservation and divergence of their functions in meiosis. Altogether, this work reveals that OsRAD51D plays an essential role in the inhibition of nonhomologous connections, thus guaranteeing faithful pairing and recombination during meiosis.Keywords
This publication has 48 references indexed in Scilit:
- Genomic features shaping the landscape of meiotic double-strand-break hotspots in maizeProceedings of the National Academy of Sciences of the United States of America, 2017
- Repair Pathway Choices and Consequences at the Double-Strand BreakTrends in Cell Biology, 2015
- Suppression of OsRAD51D results in defects in reproductive development in rice (Oryza sativa L.)The Plant Journal, 2014
- Effects of XRCC2 and RAD51B mutations on somatic and meiotic recombination in Arabidopsis thalianaThe Plant Journal, 2013
- Rad51 Paralog Complexes BCDX2 and CX3 Act at Different Stages in the BRCA1-BRCA2-Dependent Homologous Recombination PathwayMolecular and Cellular Biology, 2013
- BRCA2 acts as a RAD51 loader to facilitate telomere replication and cappingNature Structural & Molecular Biology, 2010
- Ring-shaped Rad51 Paralog Protein Complexes Bind Holliday Junctions and Replication Forks as Visualized by Electron MicroscopyPublished by Elsevier BV ,2010
- Arabidopsis SNI1 and RAD51D regulate both gene transcription and DNA recombination during the defense responseProceedings of the National Academy of Sciences of the United States of America, 2007
- Functional Interaction between the Bloom's Syndrome Helicase and the RAD51 Paralog, RAD51L3 (RAD51D)Published by Elsevier BV ,2003
- The RAD51 Family Member, RAD51L3, Is a DNA-stimulated ATPase That Forms a Complex with XRCC2Published by Elsevier BV ,2000