Anti-Biofilm Effect of Octenidine and Polyhexanide on Uropathogenic Biofilm-Producing Bacteria
Open Access
- 5 January 2021
- journal article
- research article
- Published by S. Karger AG in Urologia Internationalis
- Vol. 105 (3-4), 278-284
- https://doi.org/10.1159/000512370
Abstract
Background: A catheter allowing a release of antibacterial substances such as antiseptics into the bladder could be a new way of preventing biofilm formation and subsequent catheter-associated urinary tract infections. Methods: Minimal inhibitory and bactericidal concentration (MIC/MBC) determinations in cation-adjusted Mueller-Hinton broth and artificial urine were performed for 4 antiseptics against 3 uropathogenic biofilm producers, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Furthermore, effects of octenidine and polyhexanide against catheter biofilm formation were determined by quantification of biofilm-producing bacteria. Results: Sodium hypochlorite showed MIC/MBC values between 200 and 800 mg/L for all strains tested. Triclosan was efficient against E. coli and P. mirabilis (MIC ≤2.98 mg/L) but ineffective against P. aeruginosa. Octenidine and polyhexanide showed antibacterial activity against all 3 species tested (MIC 1.95–7.8 and 3.9–31.25 mg/L). Both octenidine and polyhexanide were able to prevent biofilm formation on catheter segments in a concentration dependent manner. Furthermore, adding 250 mg/L of each biocide disrupted biofilms formed by E. coli and P. mirabilis, whereas even 500 mg/L was not sufficient to completely destroy P. aeruginosa biofilms. Conclusion: Octenidine- and polyhexanide-containing antiseptics showed a broad effect against typical uropathogenic biofilm producers even in high dilutions. This study provides a basis for further investigation of the potential of octenidine and polyhexanide as prophylaxis or treatment of catheter biofilms.Keywords
This publication has 23 references indexed in Scilit:
- Antimicrobial Stewardship and Urinary Tract InfectionsAntibiotics, 2014
- A scoping review of important urinary catheter induced complicationsJournal of Materials Science: Materials in Medicine, 2013
- Virulence factors inProteusbacteria from biofilm communities of catheter-associated urinary tract infectionsFEMS Immunology & Medical Microbiology, 2012
- The Isolation and the Biofilm Formation of Uropathogens in the Patients with Catheter Associated Urinary Tract Infections (UTIs)Journal of Clinical and Diagnostic Research, 2012
- Evaluation of Toxic Side Effects of Clinically Used Skin Antiseptics In VitroJournal of Surgical Research, 2010
- Triclosan Resistance of Pseudomonas aeruginosa PAO1 Is Due to FabV, a Triclosan-Resistant Enoyl-Acyl Carrier Protein ReductaseAntimicrobial Agents and Chemotherapy, 2010
- Bacterial biofilms in patients with indwelling urinary cathetersNature Reviews Endocrinology, 2008
- Effect of triclosan on the development of bacterial biofilms by urinary tract pathogens on urinary cathetersJournal of Antimicrobial Chemotherapy, 2005
- Evaluation of the Antimicrobial Efficacy of Urinary Catheters Impregnated With Antiseptics in an In Vitro Urinary Tract ModelInfection Control & Hospital Epidemiology, 2003
- PRE-INOCULATION OF URINARY CATHETERS WITH ESCHERICHIA COLI 83972 INHIBITS CATHETER COLONIZATION BY ENTEROCOCCUS FAECALISJournal of Urology, 2002