Prolonging Viability of Swine Muscle Biopsy Specimens in Malignant Hyperthermia Testing
- 1 September 2001
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Anesthesia & Analgesia
- Vol. 93 (3), 781-786
- https://doi.org/10.1097/00000539-200109000-00045
Abstract
In North America, the caffeine halothane contracture test (CHCT) is the standard test for the diagnosis of malignant hyperthermia (MH). Current CHCT protocol recommends that the test be completed within 5 h of muscle excision. The purpose of this study was to investigate whether the period of skeletal muscle viability could be extended to 24 h. We tested the gracilis muscle from normal (n = 8) and MH-susceptible swine (n = 8). After baseline (1–2 h after excision) CHCT, the remaining muscles were placed into one of the following four treatment groups. In Groups 1 and 2, the muscles remained under tension and were stored in Krebs buffer (pH 7.4) at 23°C–25°C (clamped-warm) and 4°C (clamped-cold), respectively. In Groups 3 and 4, the muscle strips were dissected, and the ends were tied with silk sutures, cut from the clamp, and placed in Krebs buffer at 23°C–25°C (free-warm) and 4°C (free-cold), respectively. The responses of the treatment groups to halothane (3%) and caffeine (0.5–32 mM) were tested 22–26 h after excision. The clamped-warm storage was the only storage method to correctly diagnose MH susceptibility in all muscle strips tested. This finding was also confirmed in muscle stored under clamped-warm conditions and shipped overnight to another testing center for a parallel CHCT.This publication has 11 references indexed in Scilit:
- Between-Center Variability of Results of the In Vitro Contracture Test for Malignant Hyperthermia SusceptibilityAnesthesia & Analgesia, 2000
- In vitro contracture test for diagnosis of malignant hyperthermia following the protocol of the European MH Group: Results of testing patients surviving fulminant MH and unrelated low‐risk subjectsActa Anaesthesiologica Scandinavica, 1997
- Standardization of the Caffeine Halothane Muscle Contracture TestAnesthesia & Analgesia, 1989
- The in vitro determination of susceptibility to malignant hyperthermiaMuscle & Nerve, 1989
- Porcine malignant hyperthermia: Cell injury enhances halothane sensitivity of biopsiesMuscle & Nerve, 1986
- Effect of temperature, time and fascicle size on the caffeine contracture testCanadian Journal of Anesthesia/Journal canadien d'anesthésie, 1980
- Porcine Malignant Hyperthermia:Anesthesiology, 1975
- Binding of Radioactivity from 14C-labeled Halothane in Isolated Perfused Rat LiversAnesthesiology, 1973
- Procaine in Malignant HyperpyrexiaBMJ, 1972
- METABOLIC ERROR OF MUSCLE METABOLISM AFTER RECOVERY FROM MALIGNANT HYPERTHERMIAThe Lancet, 1970