Coat–protein–mediated resistance to tobacco mosaic virus: discovery mechanisms and exploitation
Open Access
- 29 March 1999
- journal article
- review article
- Published by The Royal Society in Philosophical Transactions Of The Royal Society B-Biological Sciences
- Vol. 354 (1383), 659-664
- https://doi.org/10.1098/rstb.1999.0418
Abstract
In 1986 we reported that transgenic plants which accumulate the coat protein of tobacco mosaic virus (TMV) are protected from infection by TMV, and by closely related tobamoviruses. The phenomenon is referred to as coat–protein–mediated resistance (CP–MR), and bears certain similarities to cross protection, a phenomenon described by plant pathologists early in this century. Our studies of CP–MR against TMV have demonstrated that transgenically expressed CP interferes with disassembly of TMV particles in the inoculated transgenic cell. However, there is little resistance to local, cell–to–cell spread of infection. CP–MR involves interaction between the transgenic CP and the CP of the challenge virus, and resistance to TMV is greater than to tobamoviruses that have CP genes more distantly related to the transgene. Using the known coordinates of the three–dimensional structure of TMV we developed mutant forms of CP that have stronger inter–subunit interactions, and confer increased levels of CP–MR compared with wild–type CP. Similarly, it is predicted that understanding the cellular and structural basis of CP–MR will lead to the development of variant CP transgenes that each can confer high levels of resistance against a range of tobamoviruses.Keywords
This publication has 29 references indexed in Scilit:
- Structure of ribgrass mosaic virus at 2.9 å resolution evolution and taxonomy of tobamovirusesJournal of Molecular Biology, 1997
- Studies of coat protein-mediated resistance to tobacco mosaic virus (TMV). II. Challenge by a mutant with altered virion surface does not overcome resistance conferred by TMV coat proteinJournal of General Virology, 1995
- Structure Determination of Cucumber Green Mottle Mosaic Virus by X-ray Fiber DiffractionJournal of Molecular Biology, 1994
- Structure of the U2 strain of tobacco mosaic virus refined at 3.5 Å resolution using X-ray fiber diffractionJournal of Molecular Biology, 1992
- Local and systemic spread of tobacco mosaic virus in transgenic tobacco.Plant Cell, 1990
- Transgenic Tobacco Plants Expressing a Coat Protein Gene of Tobacco Mosaic Virus Are Resistant to Some Other TobamovirusesMolecular Plant-Microbe Interactions®, 1990
- Plant viruses: A tool‐box for genetic engineering and crop protectionBioEssays, 1989
- Reduced Photosystem II Activity and Accumulation of Viral Coat Protein in Chloroplasts of Leaves Infected with Tobacco Mosaic VirusPlant Physiology, 1989
- Modifications of the Tobacco Mosaic Virus Coat Protein Gene Affecting Replication, Movement, and SymptomatologyPhytopathology®, 1988
- The concept of parasite-derived resistance—Deriving resistance genes from the parasite's own genomeJournal of Theoretical Biology, 1985