Protein kinase C stimulation enhances the process formation of adult oligodendrocytes and induces proliferation
- 1 August 1991
- journal article
- Published by Wiley in Journal of Neuroscience Research
- Vol. 29 (4), 481-489
- https://doi.org/10.1002/jnr.490290408
Abstract
Oligodendrocytes (OL) isolated from adult pig brains regenerate their processes and form a network of fibers after 14–18 days in vitro (DIV). Stimulation of protein kinase C (Pk‐C) by tumour promoters such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) produces at day 7 in vitro a similar network after only 20 hr. H‐7, an inhibitor of Pk‐C, as well as amiloride, which inhibits the subsequent Na+/H+ exchange, reversibly suppress this effect. Activation of the protein kinase A or calmodulin pathway do not result in an increased OL process production. Furthermore, TPA induced proliferation in a subpopulation of OL. We conclude that the stimulation of Pk‐C is of utmost importance for OL regeneration.Keywords
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