Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays
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Open Access
- 15 August 2020
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 222 (9), 1462-1467
- https://doi.org/10.1093/infdis/jiaa507
Abstract
The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels.Keywords
Funding Information
- Liverpool School of Tropical Medicine
- Biotechnology and Biological Sciences Research Council (BB/T001240/1, BB/S0011980/1)
- Royal Society Wolfson fellowship (RSWF\R1\180013)
- National Institutes of Health (R21AI138074, R21AI129507)
- UK Research and Innovation (20197)
- National Institute for Health Research (NIHR2000907)
- Medical Research Council (MR/R015406/1)
- DogsTrust
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