Different Forms of DMP1 Play Distinct Roles in Mineralization
- 3 March 2010
- journal article
- other
- Published by SAGE Publications in Journal of Dental Research
- Vol. 89 (4), 355-359
- https://doi.org/10.1177/0022034510363250
Abstract
Dentin matrix protein-1 (DMP1) is a major synthetic product of hypertrophic chondrocytes and osteocytes. Previous in vitro studies showed full-length DMP1 inhibits hydroxyapatite (HA) formation and growth, while its N-terminal fragment (37K) promotes HA formation. Since there are 3 fragments within the mineralized tissues [N-terminal, C-terminal (57K), and a chondroitin-sulfate-linked N-terminal fragment (DMP1-PG)], we predicted that each would have a distinct effect on mineralization related to its interaction with HA. In a gelatin-gel system, 37K and 57K fragments were both promoters of HA formation and growth; DMP1-PG was an inhibitor. The secondary structures of the 3 fragments and the full-length protein in the presence and absence of Ca2+ and HA determined by FTIR showed that the full-length protein undergoes slight conformational changes on binding to HA, while 37K, 57K, and DMP1-PG do not change conformation. These findings indicate that distinct forms of DMP1 may work collectively in controlling the mineralization process.Keywords
This publication has 28 references indexed in Scilit:
- MEPE-ASARM Peptides Control Extracellular Matrix Mineralization by Binding to Hydroxyapatite: An Inhibition Regulated by PHEX Cleavage of ASARMJournal of Bone and Mineral Research, 2008
- Generation of a conditional null allele for Dmp1 in mousegenesis, 2008
- Matrix Macromolecules in Hard Tissues Control the Nucleation and Hierarchical Assembly of HydroxyapatitePublished by Elsevier BV ,2007
- Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolismNature Genetics, 2006
- Spatially and Temporally Controlled Biomineralization Is Facilitated by Interaction between Self-Assembled Dentin Matrix Protein 1 and Calcium Phosphate Nuclei in SolutionBiochemistry, 2005
- Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1Nature Materials, 2003
- Dentin Matrix Protein 1 Initiates Hydroxyapatite Formation In VitroConnective Tissue Research, 2003
- Flexible Structures of SIBLING Proteins, Bone Sialoprotein, and OsteopontinBiochemical and Biophysical Research Communications, 2001
- Hydroxyapatite formation in a dynamic collagen gel system: effects of type I collagen, lipids, and proteoglycansThe Journal of Physical Chemistry, 1989
- A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphataseAnalytical Biochemistry, 1981