Upregulation of LncRNA PVT1 Facilitates Pancreatic Ductal Adenocarcinoma Cell Progression and Glycolysis by Regulating MiR-519d-3p and HIF-1A
Open Access
- 1 January 2020
- journal article
- research article
- Published by Ivyspring International Publisher in Journal of Cancer
- Vol. 11 (9), 2572-2579
- https://doi.org/10.7150/jca.37959
Abstract
The long, noncoding RNA (lncRNA) PVT1, as an important epigenetic regulator, has a critical role in carcinogenesis. However, its role in pancreatic ductal adenocarcinoma (PDAC) has not been fully investigated. Here, the up-regulated expression of lncRNA PVT1 is found in our PDAC tumor samples. Knockdown of it suppressed PDCA cells growth and glycolysis. An inverse association between miR-519d-3p and PVT1 was found. RIP, RNA pulldown and luciferase assay showed that PVT1 directly targets miR-519d-3p by binding with microRNA binding site. Bioinformatics analysis and study indicated that HIF-1A is a target of miR-519d-3p. Collectively, our findings suggested that PVT1 could act as an oncogenic lncRNA, and promote tumor progression by regulating HIF-1A via competing with miR-519d-3p.Keywords
This publication has 26 references indexed in Scilit:
- Salivary HOTAIR and PVT1 as novel biomarkers for early pancreatic cancerOncotarget, 2016
- The Effect of Silencing HIF-1α Gene in BxPC-3 Cell Line on Glycolysis-Related Gene Expression, Cell Growth, Invasion, and ApoptosisNutrition and Cancer, 2015
- From Discovery to Function: The Expanding Roles of Long NonCoding RNAs in Physiology and DiseaseEndocrine Reviews, 2015
- Long non-coding RNA PVT1 is associated with tumor progression and predicts recurrence in hepatocellular carcinoma patientsOncology Letters, 2014
- Cancer statistics, 2014CA: A Cancer Journal for Clinicians, 2014
- Long non-coding RNAs: A new frontier in the study of human diseasesCancer Letters, 2013
- Subgroup-specific structural variation across 1,000 medulloblastoma genomesNature, 2012
- A Long Noncoding RNA Controls Muscle Differentiation by Functioning as a Competing Endogenous RNACell, 2011
- Targeting HIF-1 for cancer therapyNature Reviews Cancer, 2003
- On the Origin of Cancer CellsScience, 1956