Stop-flow lithography to generate cell-laden microgel particles
- 22 May 2008
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 8 (7), 1056-1061
- https://doi.org/10.1039/b804234a
Abstract
Encapsulating cells within hydrogels is important for generating three-dimensional (3D) tissue constructs for drug delivery and tissue engineering. This paper describes, for the first time, the fabrication of large numbers of cell-laden microgel particles using a continuous microfluidic process called stop-flow lithography (SFL). Prepolymer solution containing cells was flowed through a microfluidic device and arrays of individual particles were repeatedly defined using pulses of UV light through a transparency mask. Unlike photolithography, SFL can be used to synthesize microgel particles continuously while maintaining control over particle size, shape and anisotropy. Therefore, SFL may become a useful tool for generating cell-laden microgels for various biomedical applications.This publication has 49 references indexed in Scilit:
- The influence of N‐vinyl pyrrolidone on polymerization kinetics and thermo‐mechanical properties of crosslinked acrylate polymersJournal of Polymer Science Part A: Polymer Chemistry, 2007
- Multifunctional Encoded Particles for High-Throughput Biomolecule AnalysisScience, 2007
- Direct patterning of composite biocompatible microstructures using microfluidicsLab on a Chip, 2007
- Vascularized organoid engineered by modular assembly enables blood perfusionProceedings of the National Academy of Sciences of the United States of America, 2006
- Micromolding of shape-controlled, harvestable cell-laden hydrogelsBiomaterials, 2006
- Hydrogels in Biology and Medicine: From Molecular Principles to BionanotechnologyAdvanced Materials, 2006
- Controlled Degradation and Mechanical Behavior of Photopolymerized Hyaluronic Acid NetworksBiomacromolecules, 2004
- New Directions in Photopolymerizable BiomaterialsMRS Bulletin, 2002
- Comparison of covalently and physically cross-linked polyethylene glycol-based hydrogels for the prevention of postoperative adhesions in a rat modelBiomaterials, 1995
- Tissue EngineeringScience, 1993