Superior Performance of Magnetic Nanoparticles for Entrapment and Fixation of Bovine Serum Albumin In-Vitro
Open Access
- 1 July 2020
- journal article
- research article
- Published by African Journals Online (AJOL) in Ethiopian Journal of Health Sciences
- Vol. 30 (4), 599-606
- https://doi.org/10.4314/ejhs.v30i4.15
Abstract
BACKGROUND: In recent years, extensive studies have been performed on magnetite nanoparticles (MNPs) and their applications, which have shown the current project to be one of the major applications by laboratory results. METHODS: The nanoparticles synthesized in this project were deposited by the co-precipitation method, which structure was identified by analyzers such as SEM, FT-IR, and EDX. The aim of this project is the adsorption and fixation of biomolecule (BSA (bovine serum albumin) protein on the surface of magnetic nanoparticles. RESULTS: The adsorption results by electrophoresis and spectrophotometric analyzers showed an absorption rate above 55% ie; 55% of the protein is fixed on the MNPs nanoparticles. This absorption is due to the high level of functionality of magnetic nanoparticles for adsorption of protein. The results of the EDX analysis also show the possible electrostatic bonding between the nanoparticles and the protein, this is derived from –OH with –NH2 groups of the nanobiocompound (MNPs /protein). After bonding, the two are easily separated. CONCLUSION: In this project, the Fe3O4 nanoparticles was synthesized and identified by SEM, FT-IR, and EDX analyzers and finally reacted with the BSA protein (for the absorption of protein on MNPs) under experimental conditions at a standard temperature of 25° C. The results showed that about 55% of the protein was fixed on magnetic nanoparticles.Keywords
This publication has 11 references indexed in Scilit:
- Magnetic iron oxide nanoparticles: Recent trends in design and synthesis of magnetoresponsive nanosystemsBiochemical and Biophysical Research Communications, 2015
- Synthesis methods to prepare single- and multi-core iron oxide nanoparticles for biomedical applicationsDalton Transactions, 2014
- Forensic implications of PCR inhibition—A reviewForensic Science International: Genetics, 2012
- Magnetic nanoparticles in MR imaging and drug deliveryAdvanced Drug Delivery Reviews, 2008
- PVP magnetic nanospheres: Biocompatibility, in vitro and in vivo bleomycin releaseInternational Journal of Pharmaceutics, 2007
- Yeast [PSI+] Prion Aggregates Are Formed by Small Sup35 Polymers Fragmented by Hsp104Journal of Biological Chemistry, 2003
- Preparation and characterization of amino–silane modified superparamagnetic silica nanospheresJournal of Magnetism and Magnetic Materials, 2003
- Synthesis and Self-Assembly of Au@SiO2 Core−Shell ColloidsNano Letters, 2002
- Molecular Manipulation of Microstructures: Biomaterials, Ceramics, and SemiconductorsScience, 1997
- A novel magnetic silica support for use in chromatographic and enzymatic bioprocessingBiotechnology & Bioengineering, 1991