Comparison of Immunoblotting, Calculation of the Goldmann-Witmer Coefficient, and Real-Time PCR Using Aqueous Humor Samples for Diagnosis of Ocular Toxoplasmosis
- 1 June 2008
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 46 (6), 1965-1967
- https://doi.org/10.1128/jcm.01900-07
Abstract
We compared three biological methods for the diagnosis of ocular toxoplasmosis (OT). Paired aqueous humor and serum samples from 34 patients with OT and from 76 patients with other ocular disorders were analyzed by three methods: immunoblotting or Western blotting (WB), the calculation of the Goldmann-Witmer coefficient (GWC), and PCR. WB and GWC each revealed the intraocular production of specific anti-Toxoplasma immunoglobulin G in 81% of samples (30 of 37). PCR detected toxoplasmic DNA in 38% of samples (13 of 34). Nine of the 13 PCR-positive patients were immunocompetent. Combining the techniques significantly improved the diagnostic sensitivity, to 92% for the GWC-WB combination, 90% for the WB-PCR combination, and 93% for the GWC-PCR combination. The combination of all three techniques improved the sensitivity to 97%.Keywords
This publication has 27 references indexed in Scilit:
- Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosisClinical Microbiology & Infection, 2006
- Polymerase Chain Reaction and Goldmann-Witmer Coefficient Analysis Are Complimentary for the Diagnosis of Infectious UveitisAmerican Journal of Ophthalmology, 2006
- Diagnostic Value of Specific Local Antibody Production and Nucleic Acid Amplification Technique-Nested Polymerase Chain Reaction (nPCR) in Clinically Suspected Ocular ToxoplasmosisOcular Immunology and Inflammation, 2006
- Use of PCR to diagnose Toxoplasma gondii chorioretinitis in eyes with severe vitritisClinical & Experimental Ophthalmology, 2005
- Necrotising retinopathies simulating acute retinal necrosis syndromeBritish Journal of Ophthalmology, 2005
- Aqueous Humor and Serum Immunoblotting for Immunoglobulin Types G, A, M, and E in Cases of Human Ocular ToxoplasmosisJournal of Clinical Microbiology, 2004
- Use of Fluorescence Resonance Energy Transfer Hybridization Probes To Evaluate Quantitative Real-Time PCR for Diagnosis of Ocular ToxoplasmosisJournal of Clinical Microbiology, 2004
- Comparison of Enzyme-Linked Immunosorbent Assay, Immunoblotting, and PCR for Diagnosis of Toxoplasmic ChorioretinitisJournal of Clinical Microbiology, 2003
- Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR1Note: Nucleotide sequence data reported in this paper have been submitted to GenBankTM database with the accession number AF146527 (Toxoplasma gondii genomic repetitive 529 bp fragment).1International Journal for Parasitology, 2000
- Toxoplasmic Chorioretinitis in the Setting of Acute Acquired ToxoplasmosisClinical Infectious Diseases, 1996