Fine structure of the dorsal lingual epithelium of the lizard, Gekko japonicus (Lacertilia, Gekkonidae)

Abstract

Three different types of lingual papilla were observed by scanning electron microscopy on the dorsal lingual epithelium of the lizard Gekko japonicus. Dome‐shaped lingual papillae were located at the apex. Flat, fan‐shaped lingual papillae were seen in the widest area of the lingual body. Long, scale‐like lingual papillae were arranged on the latero‐posterior dorsal surface. At higher magnification, microvilli and microridges were seen to be widely distributed over the surface of the papillae. By light microscopy, the epithelium of the dome‐shaped papillae was composed of single, columnar epithelial cells filled with secretory granules. The tip of the epithelium of the fan‐shaped and scale‐like papillae was composed of stratified squamous epithelial cells without granules. The major part of the epithelium of these two types of papilla, except the tip area, was also composed of single, columnar epithelial cells with secretory granules. By transmission electron microscopy, a nucleus without a defined shape was seen to be located in the basal part of each of the single, columnar epithelial cells. Rough‐surfaced endoplasmic reticulum and Golgi apparatus were well developed around the nucleus. The other, major part of the cytoplasm was filled with the spherical secretory granules, a large number of which had very electron‐dense cores and moderately electron‐dense peripheral regions. In the stratified squamous epithelium, a nucleus, which tended to be condensed on the free‐surface side, was located in the center of each cell. Mitochondria, endoplasmic reticulum, and vesicles were observed in the cytoplasm.