SUMO modification of a novel MAR-binding protein, SATB2, modulates immunoglobulin μ gene expression
Open Access
- 15 December 2003
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 17 (24), 3048-3061
- https://doi.org/10.1101/gad.1153003
Abstract
Nuclear matrix attachment regions (MARs) are regulatory DNA sequences that are important for higher-order chromatin organization, long-range enhancer function, and extension of chromatin modifications. Here we characterize a novel cell type-specific MAR-binding protein, SATB2, which binds to the MARs of the endogenous immunoglobulin μ locus in pre-B cells and enhances gene expression. We found that SATB2 differs from the closely related thymocyte-specific protein SATB1 by modifications of two lysines with the small ubiquitive related modifier (SUMO), which are augmented specifically by the SUMO E3 ligase PIAS1. Mutations of the SUMO conjugation sites of SATB2 enhance its activation potential and association with endogenous MARs in vivo, whereas N-terminal fusions with SUMO1 or SUMO3 decrease SATB2-mediated gene activation. Sumoylation is also involved in targeting SATB2 to the nuclear periphery, raising the possibility that this reversible modification of a MAR-binding protein may contribute to the modulation of subnuclear DNA localization.Keywords
This publication has 72 references indexed in Scilit:
- The Polycomb Protein Pc2 Is a SUMO E3Cell, 2003
- Regulating the RegulatorsCell, 2003
- RAD6-dependent DNA repair is linked to modification of PCNA by ubiquitin and SUMONature, 2002
- Subnuclear Compartmentalization of Immunoglobulin Loci During Lymphocyte DevelopmentScience, 2002
- SATB1 Cleavage by Caspase 6 Disrupts PDZ Domain-Mediated Dimerization, Causing Detachment from Chromatin Early in T-Cell ApoptosisMolecular and Cellular Biology, 2001
- SUMO-1 Modification Regulates the DNA Binding Activity of Heat Shock Transcription Factor 2, a Promyelocytic Leukemia Nuclear Body Associated Transcription FactorJournal of Biological Chemistry, 2001
- Dynamic relocation of transcription and splicing factors dependent upon transcriptional activityThe EMBO Journal, 1997
- Dependence of Enhancer-Mediated Transcription of the Immunoglobulin μ Gene on Nuclear Matrix Attachment RegionsScience, 1994
- A developmental-specific factor binds to suppressor sites flanking the immunoglobulin heavy-chain enhancer.Genes & Development, 1989
- Identification of a nuclear protein matrixBiochemical and Biophysical Research Communications, 1974