Gene expression during Drosophila melanogaster oogenesis: Analysis by in situ hybridization to tissue sections
- 30 September 1984
- journal article
- Published by Elsevier BV in Developmental Biology
- Vol. 105 (1), 80-92
- https://doi.org/10.1016/0012-1606(84)90263-x
Abstract
The pattern of gene expression for specific genes during oogenesis has been examined by in situ hybridization to RNA in sectioned ovarian preparations. The accumulation (1) of RNA complementary to heat-shock genes, both before and after heat shock; (2) of RNA complementary to three λ phage recombinants which contain sequences expressed during oogenesis; and (3) of RNA complementary to the rudimentary gene have been analyzed. Gene expression in the ovaries is found to be dependent on both the cell type and the developmental stage of the ovarian chamber. In the nurse cells, the onset of vitellogenesis (stages 8–11) appears to be accompanied by a substantial activation of many different genes and there is a pronounced increase in the level of accumulation of complementary RNAs. Moreover, much of the RNA synthesized in nurse cells during vitellogenesis is ultimately transported into the growing oocyte where it accumulates. It is likely that this accumulation of RNA provides a source of gene products, either as message or proteins, for embryogenesis.Keywords
This publication has 23 references indexed in Scilit:
- Ecdysterone induces the transcription of four heat-shock genes in Drosophila S3 cells and imaginal discsDevelopmental Biology, 1982
- Varying patterns of protein synthesis in Drosophila during heat shock: Implications for regulationDevelopmental Biology, 1980
- The induction of gene activity in drosophila by heat shockCell, 1979
- Loss of centrioles and polyploidization in follicle cells of Drosophila melanogasterExperimental Cell Research, 1979
- The isolation of structural genes from libraries of eucaryotic DNACell, 1978
- Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.Journal of Histochemistry & Cytochemistry, 1978
- Rudimentary locus of Drosophila melanogaster: Partial purification of a carbamylphosphate synthase-aspartate transcarbamylase-dihydroorotase complexBiochemical Genetics, 1978
- Yolk proteins in Drosophila: Identification and site of synthesisJournal of Experimental Zoology, 1974
- DNA SYNTHESIS IN THE OOPLASM OF DROSOPHILA MELANOGASTER The Journal of cell biology, 1966
- Cell function in the ovary of drosophilaChromosoma, 1959