Apple Proliferation Resistance in Apomictic Rootstocks and Its Relationship to Phytoplasma Concentration and Simple Sequence Repeat Genotypes
Open Access
- 1 February 2008
- journal article
- research article
- Published by Scientific Societies in Phytopathology®
- Vol. 98 (2), 153-158
- https://doi.org/10.1094/phyto-98-2-0153
Abstract
In an effort to select and characterize apple rootstock resistant to apple proliferation (AP), progenies from seven apomictic rootstock selections and their parental apomictic species, Malus sieboldii and M. sargentii, were compared to standard stocks M 9 and M 11. Seedlings derived from open pollinated mother plants were grafted with cv. Golden Delicious and grown under natural infection conditions. The progenies differed greatly in resistance to the AP agent ‘Candidatus Phytoplasma mali’. Progenies of M. sieboldii and its descendent rootstock selections D2212, 4608, 4551, and D1131 showed a high level of resistance, whereas progenies of M. sargentii and its descendent selections D1111 and C1828 proved susceptible. M 9 and M 11 showed an intermediate level of resistance. Phytoplasma titer in roots of the M. sieboldii and M. sargentii progeny groups was similarly low, whereas the concentration in the standard stocks was 100 to 5,000 times higher. In trees on most of the resistant stocks, only a minority was colonized in the scion, while in trees on susceptible and standard stocks, infection rate was often higher. Also, the titer in the top of trees on resistant stocks was usually lower than in trees on susceptible and standard stocks. Four progenies derived from open pollinated M. sieboldii and M. sieboldii descendents were subjected to DNA typing using simple sequence repeat (SSR) markers. This study revealed that the selected groups consisted mainly of mother-like plants (apomicts) and type I hybrids (unreduced mother genotype plus one male allele at each locus). Type II hybrids (full recombinants) and autopollinated offspring were rare. In the 4608 progeny, trees grown on type I hybrid rootstocks were significantly less affected than trees on mother-like stocks. In other progenies with fewer or no type I hybrids, trees on type II hybrids and autopollinated offspring suffered considerably more from disease than trees on mother-like stocks.Keywords
This publication has 18 references indexed in Scilit:
- Differences in Virulence and Genomic Features of Strains of ‘Candidatus Phytoplasma mali’, the Apple Proliferation AgentPhytopathology®, 2007
- ‘Candidatus Phytoplasma mali’, ‘Candidatus Phytoplasma pyri’ and ‘Candidatus Phytoplasma prunorum’, the causal agents of apple proliferation, pear decline and European stone fruit yellows, respectivelyInternational Journal of Systematic and Evolutionary Microbiology, 2004
- First Report of Cacopsylla picta as a Vector of Apple Proliferation Phytoplasma in GermanyPlant Disease, 2003
- Cacopsylla costalis (Flor 1861), as a Vector of Apple Proliferation in TrentinoJournal of Phytopathology, 2000
- Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus×domestica borkh. core subset collectionTheoretical and Applied Genetics, 1998
- Microsatellites in Malus X domestica (apple): abundance, polymorphism and cultivar identificationTheoretical and Applied Genetics, 1997
- Detection of mycoplasmalike organisms in declining oaks by polymerase chain reactionForest Pathology, 1994
- Susceptibility of Grafted Malus Taxa and Hybrids to Apple Proliferation DiseaseJournal of Phytopathology, 1991
- Histopathology of Apple Proliferation in Malus Taxa and Hybrids of Different SusceptibilityJournal of Phytopathology, 1991
- Condition of the Phloem and the Persistence of Mycoplasmalike Organisms Associated with Apple Proliferation and Pear DeclinePhytopathology®, 1982