Chromatin decondensation is sufficient to alter nuclear organization in embryonic stem cells
- 5 December 2014
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 346 (6214), 1238-1242
- https://doi.org/10.1126/science.1259587
Abstract
During differentiation, thousands of genes are repositioned toward or away from the nuclear envelope. These movements correlate with changes in transcription and replication timing. Using synthetic (TALE) transcription factors, we found that transcriptional activation of endogenous genes by a viral trans-activator is sufficient to induce gene repositioning toward the nuclear interior in embryonic stem cells. However, gene relocation was also induced by recruitment of an acidic peptide that decondenses chromatin without affecting transcription, indicating that nuclear reorganization is driven by chromatin remodeling rather than transcription. We identified an epigenetic inheritance of chromatin decondensation that maintained central nuclear positioning through mitosis even after the TALE transcription factor was lost. Our results also demonstrate that transcriptional activation, but not chromatin decondensation, is sufficient to change replication timing.Keywords
This publication has 24 references indexed in Scilit:
- Building silent compartments at the nuclear periphery: a recurrent themeCurrent Opinion in Genetics & Development, 2013
- Single-Cell Dynamics of Genome-Nuclear Lamina InteractionsCell, 2013
- Efficient construction of sequence-specific TAL effectors for modulating mammalian transcriptionNature Biotechnology, 2011
- Molecular Maps of the Reorganization of Genome-Nuclear Lamina Interactions during DifferentiationMolecular Cell, 2010
- Global Reorganization of Replication Domains During Embryonic Stem Cell DifferentiationPLoS Biology, 2008
- Domain organization of human chromosomes revealed by mapping of nuclear lamina interactionsNature, 2008
- Recruitment to the Nuclear Periphery Can Alter Expression of Genes in Human CellsPLoS Genetics, 2008
- Transcriptional repression mediated by repositioning of genes to the nuclear laminaNature, 2008
- Genome-wide maps of chromatin state in pluripotent and lineage-committed cellsNature, 2007
- The nuclear envelope and transcriptional controlNature Reviews Genetics, 2007