An electrorotation technique for measuring the dielectric properties of cells with simultaneous use of negative quadrupolar dielectrophoresis and electrorotation
- 1 January 2013
- journal article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 138 (5), 1529-1537
- https://doi.org/10.1039/c3an36261b
Abstract
This paper presents an effective electrorotation technique for measuring the dielectric properties of cells using a superposed electrical signal, which can simultaneously generate negative quadrupolar dielectrophoretic (nQDEP) force and electrorotational (ROT) torque. The proposed technique involves a three-dimensional (3D) octode, which includes four electrodes arranged in a crisscross pattern on the top and bottom of a microchannel, respectively. A single cell was trapped in the center of the 3D octode by the nQDEP force and simultaneously rotated by the ROT torque. Using the proposed electrorotation technique, ROT spectra of human leukocyte subpopulations (T and B lymphocytes, granulocytes, and monocytes) and metastatic human breast (SkBr3) and lung (A549) cancer cell lines were accurately measured without any disturbance. Torque on the cells generated by the ROT signal was analyzed theoretically based on the single-shell dielectric model for the cells. Furthermore, the dielectric properties of the cells, such as area-specific membrane capacitance and cytoplasm conductivity, were extracted using the measured ROT spectra and the analyzed torque.Keywords
This publication has 47 references indexed in Scilit:
- Single-cell microfluidic impedance cytometry: a reviewMicrofluidics and Nanofluidics, 2010
- Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometryLab on a Chip, 2009
- Quantification of the Heterogeneity in Breast Cancer Cell Lines Using Whole-Cell Impedance SpectroscopyClinical Cancer Research, 2007
- Single cell dielectric spectroscopyJournal of Physics D: Applied Physics, 2006
- Ion channel characterization using single cell impedance spectroscopyLab on a Chip, 2006
- Impedance spectroscopy flow cytometry: On‐chip label‐free cell differentiationCytometry Part A, 2005
- Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizingLab on a Chip, 2001
- Measurements of the dielectric properties of peripheral blood mononuclear cells and trophoblast cells using AC electrokinetic techniquesBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 2000
- Cell Separation by Dielectrophoretic Field-flow-fractionationAnalytical Chemistry, 2000
- Electric impedance spectroscopy using microchannels with integrated metal electrodesJournal of Microelectromechanical Systems, 1999