Induction of 2n female gametes in Populus adenopoda Maxim by high temperature exposure during female gametophyte development
Open Access
- 1 January 2013
- journal article
- Published by Japanese Society of Breeding in Breeding Science
- Vol. 63 (1), 96-103
- https://doi.org/10.1270/jsbbs.63.96
Abstract
In order to produce triploid plants, 2n female gametes were induced by treating female buds and developing embryo sacs of Populus adenopoda Maxim with high temperature exposure. During megasporogenesis, tests were conducted on the relationship between female gametophyte development and morphological changes of female catkins. In the resulting progeny, 12 triploids were produced, and the highest rate of triploid production was 40%. Cytological observation revealed that the pachytene to diakinesis phase of meiotic stages may be a suitable period for inducing megaspore chromosome doubling through high temperature exposure. On the other hand, catkins of 6–72 h after pollination were treated for inducing embryo sac chromosome doubling. In the offspring seedlings, 51 triploids were detected and the highest efficiency of triploid production was 83.33%. Correlation analysis between the proportion of each embryo sac’s developmental stage and the percentage of triploid production indicated that the second mitotic division may be the most effective stage for 2n female gamete induction. Our findings showed that high temperature exposure is an ideal method for 2n female gamete induction. Heterozygous offspring are valuable for breeding programs of P. adenopoda.Keywords
This publication has 16 references indexed in Scilit:
- Induction of unreduced megaspores with high temperature during megasporogenesis in PopulusAnnals of Forest Science, 2011
- Heat tolerance in plants: An overviewEnvironmental and Experimental Botany, 2007
- Chromosome numbers of some Populus taxa from ChinaActa Phytotaxonomica Sinica, 2005
- Creating triploid germplasm via induced 2n pollen inCapsicum annuumL.The Journal of Horticultural Science and Biotechnology, 2003
- Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plantsNew Phytologist, 1995
- Diploid and Polyploid Gametes in Crop Plants: Mechanisms of Formation and Utilization in Plant BreedingPublished by Wiley ,1985
- Rapid Flow Cytometric Analysis of the Cell Cycle in Intact Plant TissuesScience, 1983
- First division restitution gametes through fertile desynaptic mutants of potatoEuphytica, 1983
- The significance of 2N gametes in potato breedingTheoretical and Applied Genetics, 1977
- Some Effects of High Temperature on Polyploidy and Other Variations in MaizeProceedings of the National Academy of Sciences of the United States of America, 1932