Green Synthesis of Red‐Emitting Carbon Nanodots as a Novel “Turn‐on” Nanothermometer in Living Cells
- 24 August 2016
- journal article
- research article
- Published by Wiley in Chemistry – A European Journal
- Vol. 22 (41), 14475-14479
- https://doi.org/10.1002/chem.201602795
Abstract
Temperature measurements in biology and medical diagnostics, along with sensitive temperature probing of living cells, is of great importance; however, it still faces significant challenges. Herein, a novel “turn-on” carbon-dot-based fluorescent nanothermometry device for spatially resolved temperature measurements in living cells is presented. The carbon nanodots (CNDs) are prepared by a green microwave-assisted method and exhibit red fluorescence (λem=615 nm) with high quantum yields (15 %). Then, an on–off fluorescent probe is prepared for detecting glutathione (GSH) based on aggregation-induced fluorescence quenching. Interestingly, the quenched fluorescence could be recovered by increasing temperature and the CNDs–GSH mixture could behave as an off–on fluorescent probe for temperature. Thus, red-emitting CNDs can be utilized for “turn-on” fluorescent nanothermometry through the fluorescence quenching and recovery processes, respectively. We employ MC3T3-E1 cells as an example model to demonstrate the red-emitting CNDs can function as “non-contact” tools for the accurate measurement of temperature and its gradient inside a living cell.Keywords
Funding Information
- National Natural Science Foundation of China (51503085, 51432006)
- Natural Science Foundation of Jiangsu Province, China (BK20140157, sklssm201621)
- Public Health Research Center at Jiangnan University (JUPH201506)
- Programme of Introducing Talents of Discipline to Universities (B13025)
- 2015 postgraduate practice innovation project of ordinary college in Jiangsu (SJLX15_0552)
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