Yeast Rad51 Recombinase Mediates Polar DNA Strand Exchange in the Absence of ATP Hydrolysis
Open Access
- 1 November 1996
- journal article
- Published by Elsevier BV
- Vol. 271 (45), 27983-27986
- https://doi.org/10.1074/jbc.271.45.27983
Abstract
Saccharomyces cerevisiae RAD51 gene is required for genetic recombination and recombinational repair of DNA strand breaks. Rad51 protein has a DNA-dependent ATPase activity, and it catalyzes ATP-dependent pairing and strand exchange between homologous DNA molecules. We show here that the rad51 Arg-191 protein, which is devoid of ATPase activity, mediates the pairing and strand exchange reaction upon binding ATP. In addition, the wild type Rad51 protein can catalyze pairing and strand exchange in the presence of the nonhydrolyzable ATP analogues adenylyl-imidodiphosphate and adenosine 5′-O-thiotriphosphate. Thus, homologous pairing and the unidirectional transfer of greater than 5 kilobases of DNA can occur efficiently without the need for nucleotide hydrolysis. Consistent with the results from the biochemical analyses, expression of the rad51 Arg-191 protein in a rad51 null mutant confers normal cellular resistance to the DNA damaging agent methylmethane sulfonate, suggesting that nucleotide binding by Rad51 is sufficient for biological function.Keywords
This publication has 16 references indexed in Scilit:
- DNA Strand Exchange Promoted by RecA K72RPublished by Elsevier BV ,1996
- HOMOLOGOUS RECOMBINATION PROTEINS IN PROKARYOTES AND EUKARYOTESAnnual Review of Genetics, 1995
- DNA strand exchange mediated by a RAD51-ssDNA nucleoprotein filament with polarity opposite to that of RecACell, 1995
- Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 ProteinScience, 1994
- Functional Characterization of Residues in the P-loop Motif of the RecA Protein ATP Binding SiteJournal of Molecular Biology, 1994
- Similarity of the Yeast RAD51 Filament to the Bacterial RecA FilamentScience, 1993
- ATP hydrolysis and the displaced strand are two factors that determine the polarity of RecA-promoted DNA strand exchangeJournal of Molecular Biology, 1992
- Energetics of RecA-mediated recombination reactionsJournal of Molecular Biology, 1990
- ENZYMES OF GENERAL RECOMBINATIONAnnual Review of Biochemistry, 1987
- recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.Proceedings of the National Academy of Sciences of the United States of America, 1981