Use of an Extended Kalman Filter and development of an automated system for xylose fermentation by a recombinantEscherichia coli

Abstract

An automated system was developed for on-line monitoring and control of xylose fermentation by a recombinantEscherichia coli. A 7-L fermenter was interfaced with a personal computer. Control circuits were constructed and a software was developed to estimate the states of the fermentation using an Extended Kalman Filter. The automated system combined with the Extended Kalman Filter provided a satisfactory way to obtain on-line information regarding estimation of fermentation parameters.